Basic Vector Information
- Vector Name:
- pMJ016c
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5407 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, Roth R, Sommer F, Geimer S, Muhlhaus T, Schroda M, Goodenough U, Sti
pMJ016c vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMJ016c vector Sequence
LOCUS 40924_30925 5407 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMJ016c, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5407)
AUTHORS Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC,
Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A,
Roth R, Sommer F, Geimer S, Muhlhaus T, Schroda M, Goodenough U,
Stitt M, Griffiths H, Jonikas MC.
TITLE A repeat protein links Rubisco to form the eukaryotic
carbon-concentrating organelle
JOURNAL Proc. Natl. Acad. Sci. U.S.A. (2016) In press
PUBMED 27166422
REFERENCE 2 (bases 1 to 5407)
AUTHORS Mackinder LCM., Meyer MT, Mettler-Altmann T, Chen V, Mitchell M,
Caspari O, Freeman Rosenzweig E, Pallesen L, Reeves G, Itakura A,
Roth R, Sommer F, Geimer S, Muhlhaus T, Schroda M, Goodenough U,
Stitt M, Griffiths H, Jonikas MC.
TITLE Direct Submission
JOURNAL Submitted (13-APR-2016) Department of Plant Biology, Carnegie
Institution of Washington, 260 Panama Street, Stanford, CA 94305,
USA
REFERENCE 3 (bases 1 to 5407)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5407)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
Acad. Sci. U.S.A. (2016) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-APR-2016) Department of Plant Biology, Carnegie Institution of
Washington, 260 Panama Street, Stanford, CA 94305, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5407
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 552..1140
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 1428..1449
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 1464..1494
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1502..1518
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 1526..1542
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3857..3873)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 4086..4541
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4823..4927
/label=AmpR promoter
CDS join(4928..5407,1..378)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
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