Basic Vector Information
- Vector Name:
- pMHCluc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10322 bp
- Type:
- Shuttle vector
- Replication origin:
- ori
- Source/Author:
- van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ, Atsma DE.
pMHCluc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMHCluc vector Sequence
LOCUS 40924_30810 10322 bp DNA circular SYN 18-DEC-2018
DEFINITION Shuttle vector pMHCluc, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10322)
AUTHORS van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der
Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ,
Atsma DE.
TITLE Fibroblasts from human postmyocardial infarction scars acquire
properties of cardiomyocytes after transduction with a recombinant
myocardin gene
JOURNAL FASEB J. 21 (12), 3369-3379 (2007)
PUBMED 17579192
REFERENCE 2 (bases 1 to 10322)
AUTHORS van Tuyn J.
TITLE Direct Submission
JOURNAL Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300
RC, Netherlands
REFERENCE 3 (bases 1 to 10322)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10322)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J.";
date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC,
Netherlands"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10322
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(705..716)
/codon_start=1
/label=WELQut site
/note="WELQut protease recognition and cleavage site"
/translation="WELQ"
CDS 5592..7241
/codon_start=1
/label=luciferase
/note="firefly luciferase"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal complement(7285..7406)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(7825..8413)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8587..9444)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(9445..9549)
/label=AmpR promoter
rep_origin 9576..10000
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 10162..10210
/label=poly(A) signal
/note="synthetic polyadenylation signal"
misc_feature 10224..10315
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
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