Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V004763 | pMESy4 | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
- Vector Name:
- pMESy4
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4513 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Pardon E, Steyaert J.
pMESy4 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMESy4 vector Sequence
LOCUS 40924_30640 4513 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMESy4, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4513)
AUTHORS Pardon E, Steyaert J.
TITLE A general protocol for the generation of Nanobodies for structural
biology
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4513)
AUTHORS Pardon E, Steyaert J.
TITLE Direct Submission
JOURNAL Submitted (16-JUL-2013) Structural Biology Brussels, Vrije
Universiteit Brussel, Pleinlaan 2, Brussels 1050, Belgium
REFERENCE 3 (bases 1 to 4513)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4513)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(16-JUL-2013) Structural Biology Brussels, Vrije Universiteit
Brussel, Pleinlaan 2, Brussels 1050, Belgium"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..4513
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 107..128
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
regulatory 211..215
/regulatory_class="ribosome_binding_site"
sig_peptide 225..290
/label=pelB signal sequence
/note="leader peptide for secretion"
CDS 340..357
/label=6xHis
/note="6xHis affinity tag"
misc_feature 358..369
/label=CaptureSelect C-tag
/note="CaptureSelect C-tag"
misc_feature 373..390
/label=HA-tag
/note="HA-tag"
gene 403..1636
/gene="M13 geneIII"
/label=M13 geneIII
primer_bind complement(1642..1658)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 1871..2326
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2608..2712
/label=AmpR promoter
CDS 2713..3570
/label=AmpR
/note="beta-lactamase"
rep_origin 3744..4332
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"