Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V004765 | pMES4 | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
- Vector Name:
- pMES4
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4501 bp
- Type:
- Phage display vector
- Replication origin:
- ori
- Source/Author:
- Pellis M, Pardon E, Muyldermans S.
- Copy Number:
- High copy number
- Promoter:
- lac promoter
- Cloning Method:
- Enzyme Cut
- 3' Primer:
- M13 fwd
- Fusion Tag:
- M13 geneIII
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pMES4 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMES4 vector Sequence
LOCUS 40924_30630 4501 bp DNA circular SYN 18-DEC-2018
DEFINITION Bacterial selection and production vector pMES4, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4501)
AUTHORS Pellis M, Pardon E, Muyldermans S.
TITLE Direct Submission
JOURNAL Submitted (09-SEP-2009) Cellular and Molecular Immunology, VUB-VIB,
Pleinlaan 2, Brussels, Brussels 1050, Belgium
REFERENCE 2 (bases 1 to 4501)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 4501)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(09-SEP-2009) Cellular and Molecular Immunology, VUB-VIB, Pleinlaan
2, Brussels, Brussels 1050, Belgium"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4501
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 107..128
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
regulatory 211..215
/regulatory_class="ribosome_binding_site"
sig_peptide 225..290
/label=pelB signal sequence
/note="leader peptide for secretion"
CDS 340..357
/label=6xHis
/note="6xHis affinity tag"
misc_feature 361..390
/label=HA-tag
/note="HA-tag"
gene 391..1623
/gene="M13 geneIII"
/label=M13 geneIII
primer_bind complement(1630..1646)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 1859..2314
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2596..2700
/label=AmpR promoter
CDS 2701..3558
/label=AmpR
/note="beta-lactamase"
rep_origin 3732..4320
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"