pME8 vector (V004772)

LOCUS       40924_30575        7235 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pME8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Audtho M, Ratlertkarn M, Wiwat C.
  TITLE     pME8, an improved shuttle vector for expression in Bacillus spp. and
            E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Audtho M, Ratlertkarn M, Wiwat C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2013) National Center for Genetic Engineering and 
            Biotechnology, National Science and Technology Development Agency, 
            113 Phaholyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120,
            Thailand
REFERENCE   3  (bases 1 to 7235)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7235)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-FEB-2013) National Center for Genetic Engineering and 
            Biotechnology, National Science and Technology Development Agency, 
            113 Phaholyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120,
            Thailand"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..105
                     /note="contains cloning sites SmaI, PstI, EcoRI, EcoRV,
                     HindIII, BglII, SalI, NotI, SacI, SacII, PstI, XbaI, XhoI, 
                     ApaI, and KpnI"
     promoter        complement(118..136)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(157..173)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(181..197)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(205..235)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(250..271)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(559..1147)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1321..2178)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2179..2283)
                     /label=AmpR promoter
     rep_origin      2310..2765
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2906..2922
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2929..2947
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_difference 3389
                     /replace="g"
                     /label=G > C to delete EcoRI site in pBC16
                     /note="G > C to delete EcoRI site in pBC16"
     CDS             3807..4808
                     /label=repB
                     /note="RepB replication protein"
     CDS             5011..6384
                     /label=TcR
                     /note="tetracycline efflux protein"
     misc_difference 6445
                     /replace="g"
                     /note="G > C to delete EcoRI site in DNA fragment from
                     pBC16"
     rep_origin      6504..6780
                     /label=ori L
                     /note="ori L"