pME18S-FL3-3 vector (V004782)

MSDS

Basic Vector Information

Vector Name:: pME18S-FL3-3

Antibiotic Resistance:: Ampicillin

Length:: 3392 bp

Type:: Expression vector

Replication origin:: ori

Source/Author:: Maruyama K, Sugano S.

Promoter:: SRα

pME18S-FL3-3 vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pME18S-FL3-3 vector Sequence

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LOCUS       40924_30520        3392 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pME18S-FL3-3 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Maruyama K, Sugano S.
  TITLE     pME18S-FL3-3: a versatile expression vector
  JOURNAL   Published Only in Database (1997)
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Maruyama K, Sugano S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-1997) Sumio Sugano, The Institute of Medical 
            Science, University of Tokyo, Department of Virology; 4-6-1, 
            Shirokanedai, Minatoku, Tokyo 108, Japan 
            (E-mail:ssugano@ims.u-tokyo.ac.jp, Tel:81-3-5449-5286, 
            Fax:81-3-5449-5416)
REFERENCE   3  (bases 1 to 3392)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3392)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (1997)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-DEC-1997) Sumio Sugano, The Institute of Medical Science, 
            University of Tokyo, Department of Virology"; volume: " 4-6-1, 
            Shirokanedai, Minatoku, Tokyo 108, Japan 
            (E-mail:ssugano@ims.u-tokyo.ac.jp, Tel:81-3-5449-5286, Fax"; pages: 
            "81-3-5449-5416"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On May 9, 2005 this sequence version replaced AB009864.1.
FEATURES             Location/Qualifiers
     source          1..3392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..632
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     intron          662..755
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    1297..1431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1641..2229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2403..3260)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3261..3365)
                     /label=AmpR promoter

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