Basic Vector Information
- Vector Name:
- pME-loxP-mCherry-pA-loxP
- Antibiotic Resistance:
- Kanamycin
- Length:
- 3554 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Sztal TE, Zhao M, Williams C, Oorschot V, Parslow AC, Giousoh A, Yuen M, Hall TE, Costin A, Ramm G, Bird PI, Busch-Nentwich EM, Stemple DL, Currie PD, Cooper ST, Laing NG, Nowak KJ, Bryson-Richardson
pME-loxP-mCherry-pA-loxP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pME-loxP-mCherry-pA-loxP vector Sequence
LOCUS 40924_30505 3554 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pME-loxP-mCherry-pA-loxP, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3554)
AUTHORS Sztal TE, Zhao M, Williams C, Oorschot V, Parslow AC, Giousoh A,
Yuen M, Hall TE, Costin A, Ramm G, Bird PI, Busch-Nentwich EM,
Stemple DL, Currie PD, Cooper ST, Laing NG, Nowak KJ,
Bryson-Richardson RJ.
TITLE Zebrafish models for nemaline myopathy reveal a spectrum of nemaline
bodies contributing to reduced muscle function
JOURNAL Acta Neuropathol. 130 (3), 389-406 (2015)
PUBMED 25931053
REFERENCE 2 (bases 1 to 3554)
AUTHORS Hall TE.
TITLE Cre-lox in zebrafish
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 3554)
AUTHORS Hall TE.
TITLE Direct Submission
JOURNAL Submitted (21-OCT-2013) Institute for Molecular Bioscience,
University of Queensland, 306 Carmody Road, St Lucia, QLD 4067,
Australia
REFERENCE 4 (bases 1 to 3554)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 3554)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Acta
Neuropathol."; date: "2015"; volume: "130"; issue: "3"; pages:
"389-406"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(21-OCT-2013) Institute for Molecular Bioscience, University of
Queensland, 306 Carmody Road, St Lucia, QLD 4067, Australia"
COMMENT SGRef: number: 4; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3554
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(268..295)
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(387..473)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 537..553
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 569..668
/label=attL1
/note="recombination site for the Gateway(R) LR reaction"
misc_recomb 669..702
/label=LoxP
/note="LoxP"
protein_bind 669..702
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS 709..1416
/codon_start=1
/label=mCherry
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
promoter complement(1435..1452)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal complement(1457..1591)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind complement(1645..1678)
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
protein_bind complement(1679..1778)
/label=attL2
/note="recombination site for the Gateway(R) LR reaction"
promoter complement(1796..1814)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1819..1835)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 1948..2754
/codon_start=1
/label=KanR
/note="aminoglycoside phosphotransferase"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 2904..3492
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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