pMDC-pRPS2-HPB vector (V004799)

LOCUS       40924_30425       12768 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway destination vector pMDC-pRPS2-HPB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12768)
  AUTHORS   Qi Y, Katagiri F.
  TITLE     Purification of low-abundance Arabidopsis plasma-membrane protein 
            complexes and identification of candidate components
  JOURNAL   Plant J. 57 (5), 932-944 (2009)
  PUBMED    19000159
REFERENCE   2  (bases 1 to 12768)
  AUTHORS   Qi Y, Katagiri F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2008) Plant Biology, University of Minnesota, 1500
            Gortner Ave., St.Paul, MN 55414, USA
REFERENCE   3  (bases 1 to 12768)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12768)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2009"; volume: "57"; issue: "5"; pages: "932-944"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-AUG-2008) Plant Biology, University of Minnesota, 1500 Gortner 
            Ave., St.Paul, MN 55414, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(6..253)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(576..599)
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             complement(609..635)
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     protein_bind    664..788
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(832..1134)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(1479..2135)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2189..2219)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(2244..2368)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      complement(2406..3859)
                     /label=RPS2 promoter
                     /note="RPS2 promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(3870..3886)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4089..4113
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5413..6039
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6476..7540
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7609..7803
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8147..8287
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8473..9061)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9151..9942)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    10367..10391
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(10469..10643)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10686..11708)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(11776..12453)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    12644..12665
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        12680..12710
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12718..12734
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12742..12758
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"