Basic Vector Information
- Vector Name:
- pMCG161
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 14105 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Sidorenko L, McGinnis K, Jorgensen R, Chandler V.
- Promoter:
- Ubi
pMCG161 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMCG161 vector Sequence
LOCUS 40924_29966 14105 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMCG161, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14105)
AUTHORS Sidorenko L, McGinnis K, Jorgensen R, Chandler V.
TITLE Vector for silencing of maize chromatin genes
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 14105)
AUTHORS Sidorenko L, McGinnis K, Jorgensen R, Chandler V.
TITLE Direct Submission
JOURNAL Submitted (12-MAR-2004) Plant Sciences, University of Arizona, 303
Forbes Hall, Tucson, AZ 85721, USA
REFERENCE 3 (bases 1 to 14105)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 14105)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(12-MAR-2004) Plant Sciences, University of Arizona, 303 Forbes
Hall, Tucson, AZ 85721, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..14105
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(28..279)
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(297..845)
/label=BlpR
/note="phosphinothricin acetyltransferase"
promoter complement(875..2865)
/label=Ubi promoter
/note="maize polyubiquitin gene promoter"
misc_feature 2882..3439
/label=small subunit ribosomal RNA (ssu) 3'
/note="small subunit ribosomal RNA (ssu) 3'"
promoter 4457..4804
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
intron 4837..5402
/number=1
/note="maize adh1"
misc_feature 5422..5441
/note="multiple cloning site; includes AscI, EcoRV, RsrII,
and AvrII; used for cloning first inverted repeat, forward
orientation"
intron 5443..6580
/number=1
/note="rice Nipponbare waxy-a"
misc_feature 6584..6604
/note="multiple cloning site; includes SgfI, SmaI, and
SpeI; used for cloning second inverted repeat, reverse
orientation"
terminator 6623..7330
/label=OCS terminator
/note="octopine synthase terminator"
primer_bind complement(7355..7371)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 7574..7598
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 8898..9524
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 9961..11025
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 11094..11288
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 11632..11772
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(11958..12546)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(12820..13476)
/label=CmR
/note="chloramphenicol acetyltransferase"
misc_feature 14004..14028
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
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