pMCG161 vector (V004808)

LOCUS       40924_29966       14105 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMCG161, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14105)
  AUTHORS   Sidorenko L, McGinnis K, Jorgensen R, Chandler V.
  TITLE     Vector for silencing of maize chromatin genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14105)
  AUTHORS   Sidorenko L, McGinnis K, Jorgensen R, Chandler V.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2004) Plant Sciences, University of Arizona, 303 
            Forbes Hall, Tucson, AZ 85721, USA
REFERENCE   3  (bases 1 to 14105)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14105)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAR-2004) Plant Sciences, University of Arizona, 303 Forbes 
            Hall, Tucson, AZ 85721, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(28..279)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(297..845)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(875..2865)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_feature    2882..3439
                     /label=small subunit ribosomal RNA (ssu) 3'
                     /note="small subunit ribosomal RNA (ssu) 3'"
     promoter        4457..4804
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     intron          4837..5402
                     /number=1
                     /note="maize adh1"
     misc_feature    5422..5441
                     /note="multiple cloning site; includes AscI, EcoRV, RsrII,
                     and AvrII; used for cloning first inverted repeat, forward 
                     orientation"
     intron          5443..6580
                     /number=1
                     /note="rice Nipponbare waxy-a"
     misc_feature    6584..6604
                     /note="multiple cloning site; includes SgfI, SmaI, and
                     SpeI; used for cloning second inverted repeat, reverse 
                     orientation"
     terminator      6623..7330
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(7355..7371)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7574..7598
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8898..9524
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             9961..11025
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      11094..11288
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    11632..11772
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(11958..12546)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12820..13476)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     misc_feature    14004..14028
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"