Basic Vector Information
- Vector Name:
- pMC1neo-polyA
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3857 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Marsh S.
pMC1neo-polyA vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMC1neo-polyA vector Sequence
LOCUS 40924_29916 3857 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pMC1neo-polyA, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3857) AUTHORS Marsh S. TITLE Direct Submission JOURNAL Submitted (19-DEC-1995) Sam Marsh, Marketing, Stratagene, 11011 North Torrey Pines Road, La Jolla, CA 92037, USA REFERENCE 2 (bases 1 to 3857) TITLE Direct Submission REFERENCE 3 (bases 1 to 3857) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted (19-DEC-1995) Sam Marsh, Marketing, Stratagene, 11011 North Torrey Pines Road, La Jolla, CA 92037, USA" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..3857 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 142..280 /label=bom /note="basis of mobility region from pBR322" misc_feature 455..731 /label=TK promoter /note="TK promoter" CDS 732..1532 /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase from Tn5" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 1542..1590 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" primer_bind complement(1629..1645) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1653..1669) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1677..1707) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(1722..1743) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(2030..2618) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2792..3649) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3650..3754) /label=AmpR promoter
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