Basic Vector Information
- Vector Name:
- pMA_Level1C
- Antibiotic Resistance:
- Kanamycin
- Length:
- 3105 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Andreou AI, Nakayama N.
pMA_Level1C vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMA_Level1C vector Sequence
LOCUS 40924_29851 3105 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMA_Level1C, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3105)
AUTHORS Andreou AI, Nakayama N.
TITLE Mobius Assembly: A versatile Golden-Gate framework towards universal
DNA assembly
JOURNAL PLoS ONE 13 (1), e0189892 (2018)
PUBMED 29293531
REFERENCE 2 (bases 1 to 3105)
AUTHORS Andreou AI, Nakayama N.
TITLE Direct Submission
JOURNAL Submitted (20-OCT-2017) Centre for Synthetic and Systems Biology and
Institute of Molecular Plant Sciences, University of Edinburgh, Max
Born Crescent, Kings Buildings, Edinburgh EH9 3BF, UK
REFERENCE 3 (bases 1 to 3105)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3105)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2018"; volume: "13"; issue: "1"; pages: "e0189892"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(20-OCT-2017) Centre for Synthetic and Systems Biology and Institute
of Molecular Plant Sciences, University of Edinburgh, Max Born
Crescent, Kings Buildings, Edinburgh EH9 3BF, UK"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..3105
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator 2..59
/label=his operon terminator
/note="This putative transcriptin terminator from the E.
coli his operon has a 2-bp deletion introduced during
synthesis. Its efficiency has not been determined."
rep_origin complement(254..842)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator complement(1025..1119)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(1143..1955)
/label=KanR
/note="aminoglycoside phosphotransferase"
promoter complement(1956..2059)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
terminator complement(2139..2182)
/label=bacterial terminator
/note="putative bacterial transcription terminator"
misc_feature 2202..2205
/label=GTCA overlap
/note="GTCA overlap"
misc_feature 2206..2209
/label=GGAG overlap
/note="GGAG overlap"
regulatory 2217..2251
/label=Anderson J23106 promoter
/note="Anderson J23106 promoter"
/regulatory_class="promoter"
regulatory 2260..2271
/label=B0034 RBS
/note="B0034 RBS"
/regulatory_class="ribosome_binding_site"
RBS 2260..2271
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 2278..2949
/label=spisCP
/note="spisCP is a fluorescent protein published in 2008,
derived from Stylophora pistillata. It is reported to be a
tetramer."
terminator 2960..3031
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 3047..3074
/label=T7Te terminator
/note="phage T7 early transcription terminator"
misc_feature 3082..3085
/label=CGCT overlap
/note="CGCT overlap"
misc_feature 3086..3089
/label=CTTG overlap
/note="CTTG overlap"
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