pMA_Level1A vector (V004828)

LOCUS       40924_29841        3101 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMA_Level1A, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Andreou AI, Nakayama N.
  TITLE     Mobius Assembly: A versatile Golden-Gate framework towards universal
            DNA assembly
  JOURNAL   PLoS ONE 13 (1), e0189892 (2018)
  PUBMED    29293531
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Andreou AI, Nakayama N.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2017) Centre for Synthetic and Systems Biology and
            Institute of Molecular Plant Sciences, University of Edinburgh, Max 
            Born Crescent, Kings Buildings, Edinburgh EH9 3BF, UK
REFERENCE   3  (bases 1 to 3101)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3101)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2018"; volume: "13"; issue: "1"; pages: "e0189892"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-OCT-2017) Centre for Synthetic and Systems Biology and Institute
            of Molecular Plant Sciences, University of Edinburgh, Max Born 
            Crescent, Kings Buildings, Edinburgh EH9 3BF, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2..59
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(254..842)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(1025..1119)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1143..1955)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(1956..2059)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(2139..2182)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2202..2205
                     /label=GGAG overlap
                     /note="GGAG overlap"
     regulatory      2213..2247
                     /label=Anderson J23106 promoter
                     /note="Anderson J23106 promoter"
                     /regulatory_class="promoter"
     regulatory      2256..2267
                     /label=B0034 RBS
                     /note="B0034 RBS"
                     /regulatory_class="ribosome_binding_site"
     RBS             2256..2267
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2274..2945
                     /label=spisCP
                     /note="spisCP is a fluorescent protein published in 2008, 
                     derived from Stylophora pistillata. It is reported to be a 
                     tetramer."
     terminator      2956..3027
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3043..3070
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    3078..3081
                     /label=CGCT overlap
                     /note="CGCT overlap"
     misc_feature    3082..3085
                     /label=CAGA overlap
                     /note="CAGA overlap"