Basic Vector Information
- Vector Name:
- pMAM
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7632 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Kitts PA.
pMAM vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMAM vector Sequence
LOCUS 40924_29781 7632 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMAM, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7632)
AUTHORS Kitts PA.
TITLE CLONTECH Vectors On Disc version 1.3
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7632)
AUTHORS Kitts PA.
TITLE Direct Submission
JOURNAL Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc.,
1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE 3 (bases 1 to 7632)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7632)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East
Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT This vector can be obtained from CLONTECH Laboratories, Inc., 1020
East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
(415) 424-8222 or (800) 662-2566, extension 1. International
customers, please contact your local distributor. For technical
information, call (415) 424- 8222 or (800) 662-2566, extension 3.
This sequence has been compiled from information in the sequence
databases, published literature and other sources, together with
partial sequences obtained by CLONTECH; this vector has not been
completely sequenced. If you suspect there is an error in this
sequence, please contact CLONTECH's Technical Service Department at
(415) 424-8222 or (800) 662-2566, extension 3 or E-mail
TECH@CLONTECH.COM.
FEATURES Location/Qualifiers
source 1..7632
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 9..1320
/label=MMTV
/note="Mouse mammary tumor virus long terminal repeat
(LTR)"
intron 1562..1627
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 1757..1777
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 2202..2336
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 2349..2678
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 2878..3333
/gene="gpt"
/label=gpt
/note="Xanthine-guanine phosphoribosyltransferase from
Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC).
Accession#: B7N8G9"
intron 3813..3878
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4008..4028
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 4453..4587
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 5440..5544
/label=AmpR promoter
CDS 5545..6402
/label=AmpR
/note="beta-lactamase"
rep_origin 6576..7164
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(7350..7490)
/label=bom
/note="basis of mobility region from pBR322"
This page is informational only.