Basic Vector Information
- Vector Name:
- pMAM
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7632 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Kitts PA.
pMAM vector Map
pMAM vector Sequence
LOCUS V004839 7632 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004839
VERSION V004839
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 7632)
AUTHORS Kitts PA.
TITLE CLONTECH Vectors On Disc version 1.3
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7632)
AUTHORS Kitts PA.
TITLE Direct Submission
JOURNAL Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc.,
1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE 3 (bases 1 to 7632)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7632)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East
Meadow Circle, Palo Alto, CA 94303, USA"
SGRef: number: 3; type: "Journal Article"
This vector can be obtained from CLONTECH Laboratories, Inc., 1020
East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
(415) 424-8222 or (800) 662-2566, extension 1. International
customers, please contact your local distributor. For technical
information, call (415) 424- 8222 or (800) 662-2566, extension 3.
This sequence has been compiled from information in the sequence
databases, published literature and other sources, together with
partial sequences obtained by CLONTECH; this vector has not been
completely sequenced. If you suspect there is an error in this
sequence, please contact CLONTECH's Technical Service Department at
(415) 424-8222 or (800) 662-2566, extension 3 or E-mail
TECH@CLONTECH.COM.
FEATURES Location/Qualifiers
source 1..7632
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 9..1320
/label="MMTV"
/note="Mouse mammary tumor virus long terminal repeat
(LTR)"
intron 1562..1627
/label="small t intron"
/note="SV40 (simian virus 40) small t antigen intron"
CDS 1757..1777
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 2202..2336
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 2349..2678
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 2878..3333
/gene="gpt"
/label="Xanthine-guanine phosphoribosyltransferase"
/note="Xanthine-guanine phosphoribosyltransferase from
Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC).
Accession#: B7N8G9"
intron 3813..3878
/label="small t intron"
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4008..4028
/label="SV40 NLS"
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 4453..4587
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 5440..5544
/label="AmpR promoter"
CDS 5545..6402
/label="AmpR"
/note="beta-lactamase"
rep_origin 6576..7164
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(7350..7490)
/label="bom"
/note="basis of mobility region from pBR322"
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