Basic Vector Information
- Vector Name:
- pmAlbEPintPlauGH
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7538 bp
- Type:
- Mammalian expression vector
- Replication origin:
- ori
- Source/Author:
- Suemizu H, Hasegawa M, Kawai K, Taniguchi K, Monnai M, Wakui M, Suematsu M, Ito M, Peltz G, Nakamura M.
- Promoter:
- Alb
pmAlbEPintPlauGH vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pmAlbEPintPlauGH vector Sequence
LOCUS 40924_29776 7538 bp DNA circular SYN 18-DEC-2018
DEFINITION Mammalian expression vector pmAlbEPintPlauGH DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7538)
AUTHORS Suemizu H, Hasegawa M, Kawai K, Taniguchi K, Monnai M, Wakui M,
Suematsu M, Ito M, Peltz G, Nakamura M.
TITLE Establishment of a humanized model of liver using NOD/Shi-scid
IL2Rgnull mice
JOURNAL Biochem. Biophys. Res. Commun. 377 (1), 248-252 (2008)
PUBMED 18840406
REFERENCE 2 (bases 1 to 7538)
AUTHORS Suemizu H, Hasegawa M, Kawai K, Taniguchi K, Monnai M, Wakui M,
Suematsu M, Ito M, Peltz G, Nakamura M.
TITLE Expression vector pmAlbEPintPlauGH DNA, complete sequence
JOURNAL Published Only in Database (2008)
REFERENCE 3 (bases 1 to 7538)
AUTHORS Suemizu H, Hasegawa M, Kawai K, Nakamura M.
TITLE Direct Submission
JOURNAL Submitted (07-AUG-2008) Contact:Hiroshi Suemizu Central Institute
for Experimental Animals, Biomedical Research Department; 3-25-12
Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan
REFERENCE 4 (bases 1 to 7538)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 7538)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biochem.
Biophys. Res. Commun."; date: "2008"; volume: "377"; issue: "1";
pages: "248-252"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Published
Only in Database (2008)"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(07-AUG-2008) Contact:Hiroshi Suemizu Central Institute for
Experimental Animals, Biomedical Research Department; 3-25-12
Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan"
COMMENT SGRef: number: 4; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7538
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 678..3013
/label=Alb
/note="Mouse albumin promoter"
intron 3130..3262
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
promoter 3307..3325
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 3340..4638
/gene="Plau"
/label=Plau
/note="Urokinase-type plasminogen activator from Mus
musculus. Accession#: P06869"
polyA_signal 4694..5316
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
promoter complement(5350..5368)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5389..5405)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5413..5429)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5437..5467)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5482..5503)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5791..6379)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6553..7410)
/label=AmpR
/note="beta-lactamase"
promoter complement(7411..7515)
/label=AmpR promoter
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