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pMAD2 vector (V004847)

Basic Vector Information

Vector Name:
pMAD2
Antibiotic Resistance:
Ampicillin
Length:
9670 bp
Type:
Cloning vector
Replication origin:
ori
Source/Author:
Bonnin RA, Le Lam TN, Bohn C, Bouloc P.

pMAD2 vector Vector Map

pMAD29670 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600AmpR promoterermCprebgaBropbomoriAmpR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMAD2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_29661        9670 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAD2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   Bonnin RA, Le Lam TN, Bohn C, Bouloc P.
  TITLE     Deciphering RNase III essentiality in Staphylococcus aureus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9670)
  AUTHORS   Bonnin RA, Le Lam TN, Bohn C, Bouloc P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2015) Institut de Biologie Integrative de la 
            Cellule, Faculte Paris Sud, Batiment 400, Orsay 91400, France
REFERENCE   3  (bases 1 to 9670)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9670)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JUL-2015) Institut de Biologie Integrative de la Cellule, 
            Faculte Paris Sud, Batiment 400, Orsay 91400, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /label=AmpR promoter
     CDS             complement(303..1034)
                     /gene="ermC"
                     /label=ermC
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     CDS             1446..2654
                     /gene="pre"
                     /label=pre
                     /note="Plasmid recombination enzyme type 1 from
                     Staphylococcus aureus. Accession#: P03857"
     CDS             complement(4179..6194)
                     /gene="bgaB"
                     /label=bgaB
                     /note="Beta-galactosidase bgaB from Geobacillus
                     kaustophilus. Accession#: P19668"
     CDS             7432..7620
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    7725..7865
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8051..8639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8813..9670)
                     /label=AmpR
                     /note="beta-lactamase"

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