Basic Vector Information
- Vector Name:
- pLZE1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3423 bp
- Type:
- Zeocin resistance loxP vector
- Replication origin:
- ori
- Source/Author:
- Choi KH, Mima T, Casart Y, Rholl D, Kumar A, Beacham IR, Schweizer HP.
- Promoter:
- EM7
pLZE1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLZE1 vector Sequence
LOCUS 40924_29571 3423 bp DNA circular SYN 18-DEC-2018
DEFINITION Zeocin resistance loxP vector pLZE1, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3423)
AUTHORS Choi KH, Mima T, Casart Y, Rholl D, Kumar A, Beacham IR, Schweizer
HP.
TITLE Genetic tools for select-agent-compliant manipulation of
Burkholderia pseudomallei
JOURNAL Appl. Environ. Microbiol. 74 (4), 1064-1075 (2008)
PUBMED 18156318
REFERENCE 2 (bases 1 to 3423)
AUTHORS Choi K-H., Schweizer HP.
TITLE Genetic tools for Pseudomonas and its related bacteria
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 3423)
AUTHORS Choi K-H., Schweizer HP.
TITLE Direct Submission
JOURNAL Submitted (21-SEP-2007) Microbiology, Immunology and Pathology,
Colorado State University, 1682 Campus Delivery, Ft. Collins, CO
80523-1682, USA
REFERENCE 4 (bases 1 to 3423)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 3423)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
Environ. Microbiol."; date: "2008"; volume: "74"; issue: "4"; pages:
"1064-1075"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(21-SEP-2007) Microbiology, Immunology and Pathology, Colorado State
University, 1682 Campus Delivery, Ft. Collins, CO 80523-1682, USA"
COMMENT SGRef: number: 4; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3423
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(184..772)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(946..1803)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1804..1908)
/label=AmpR promoter
primer_bind 2380..2396
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 2447..2479
/label=5' loxP site
/note="5' loxP site"
promoter 2597..2644
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 2663..3034
/codon_start=1
/label=BleoR
/note="antibiotic-binding protein"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
protein_bind complement(3112..3145)
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
primer_bind complement(3205..3221)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(3229..3245)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3253..3283)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3298..3319)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
This page is informational only.