Basic Vector Information
- Vector Name:
- pLZ2
- Antibiotic Resistance:
- Kanamycin
- Length:
- 9272 bp
- Type:
- Expression vector
- Replication origin:
- RSF ori
- Source/Author:
- Zhu L, Dalbey RE.
pLZ2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLZ2 vector Sequence
LOCUS 40924_29546 9272 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pLZ2, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9272)
AUTHORS Zhu L, Dalbey RE.
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2014) Department of Chemistry and Biochemistry,
The Ohio State University, RM580, W.12th Ave, Columbus, OH 43210,
USA
REFERENCE 2 (bases 1 to 9272)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 9272)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(31-MAR-2014) Department of Chemistry and Biochemistry, The Ohio
State University, RM580, W.12th Ave, Columbus, OH 43210, USA"
COMMENT SGRef: number: 2; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..9272
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 28..72
/label=S-Tag
/note="affinity and epitope tag derived from pancreatic
ribonuclease A"
terminator 124..171
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(206..2854)
/note="T7 RNA polymerase from Escherichia phage T7.
Accession#: P00573"
CDS complement(2953..3018)
/label=lambda N peptide
/note="N-terminal RNA-binding domain from the lambda
bacteriophage antiterminator protein N (Baron-Benhamou et
al., 2004)"
regulatory complement(3226..3236)
/note="pL; induces expression of T7 RNA polymerase"
/regulatory_class="promoter"
regulatory complement(3247..3252)
/label=lambda left promoter
/note="lambda left promoter"
/regulatory_class="minus_10_signal"
regulatory complement(3270..3275)
/label=lambda left promoter
/note="lambda left promoter"
/regulatory_class="minus_35_signal"
promoter 3415..3445
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
protein_bind 3453..3469
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 3490..4200
/label=lambda repressor (ts)
/note="temperature-sensitive variant of the phage lambda
repressor"
CDS complement(4656..5468)
/label=KanR
/note="aminoglycoside phosphotransferase"
promoter complement(5469..5560)
/label=AmpR promoter
rep_origin complement(5576..6325)
/direction=LEFT
/label=RSF ori
/note="Plasmids containing the RSF 1030 origin of
replication can be propagated in E. coli cells that contain
additional plasmids with compatible origins."
protein_bind complement(6487..6508)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(6524..7603)
/label=lacI
/note="lac repressor"
promoter complement(7604..7680)
/label=lacI promoter
promoter 7698..7716
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 7781..7802
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7817..7847
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7855..7871
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7879..7895
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7911..7927)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
regulatory 8007..8013
/regulatory_class="ribosome_binding_site"
CDS 8021..9271
/gene="lacY"
/label=lacY
/note="Lactose permease from Escherichia coli (strain K12).
Accession#: P02920"
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