pLYS1K vector (V004870)

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LOCUS       40924_29521        5545 bp DNA     circular SYN 18-DEC-2018
DEFINITION  S. pombe expression vector pLYS1K DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     A novel series of vectors for chromosomal integration in fission 
            yeast
  JOURNAL   Biochem. Biophys. Res. Commun. 374 (2), 315-319 (2008)
  PUBMED    18634753
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Fission yeast integration vector pLYS1K
  JOURNAL   Published Only in Database (2007)
REFERENCE   3  (bases 1 to 5545)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical 
            Genetics Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL
            :http://cgl.riken.go.jp/
REFERENCE   4  (bases 1 to 5545)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5545)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2008"; volume: "374"; issue: "2"; 
            pages: "315-319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2007)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical Genetics 
            Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL 
            :http://cgl.riken.go.jp/"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     Fission yeast expression vector.
FEATURES             Location/Qualifiers
     source          1..5545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..208
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    242..956
                     /pseudo
                     /gene="lys1"
                     /label=derived from Schizosaccharomyces pombe
                     /note="derived from Schizosaccharomyces pombe"
     CDS             242..956
                     /pseudo
                     /codon_start=1
                     /gene="lys1"
                     /label=lys1
                     /note="NotI site is created at the middle of this fragment 
                     (613-620)."
                     /translation="VLCSTCAVSRSNIFGIAKRCHSSRTWHEECPTFGDQSF*Y*QDMR
                     YRRSW*NISSCWWIG*GLPWK**ID*QKILKELVC*SLQVCRPNSRKCALETLLVRNTR
                     SYVSFW*LGPLSPNW*CRM*RPPTIKLKFVVSELS*VKLIPIFLVILMSVKILPLFDVI
                     RMKNPR*LHTLYLKV*IKMTLIALLSRKILSSMV*RSTGSLYMIYGNILRLNFLVMLFL
                     L*LFPFIKCLSILMER"
     gene            242..956
                     /pseudo
                     /gene="lys1"
                     /label=lys1
     gene            998..2354
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     primer_bind     complement(2405..2421)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2634..3089
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3371..3475
                     /label=AmpR promoter
     CDS             3476..4333
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4507..5095
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5383..5404
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5419..5449
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5457..5473
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5481..5497
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"