Basic Vector Information
- Vector Name:
- pLYS1B
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5347 bp
- Type:
- S. pombe expression vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Matsuyama A, Shirai A, Yoshida M.
- Promoter:
- TEF1
pLYS1B vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLYS1B vector Sequence
LOCUS 40924_29511 5347 bp DNA circular SYN 18-DEC-2018
DEFINITION S. pombe expression vector pLYS1B DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5347)
AUTHORS Matsuyama A, Shirai A, Yoshida M.
TITLE A novel series of vectors for chromosomal integration in fission
yeast
JOURNAL Biochem. Biophys. Res. Commun. 374 (2), 315-319 (2008)
PUBMED 18634753
REFERENCE 2 (bases 1 to 5347)
AUTHORS Matsuyama A, Shirai A, Yoshida M.
TITLE Fission yeast integration vector pLYS1B
JOURNAL Published Only in Database (2007)
REFERENCE 3 (bases 1 to 5347)
AUTHORS Matsuyama A, Shirai A, Yoshida M.
TITLE Direct Submission
JOURNAL Submitted (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical
Genetics Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL
:http://cgl.riken.go.jp/
REFERENCE 4 (bases 1 to 5347)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 5347)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biochem.
Biophys. Res. Commun."; date: "2008"; volume: "374"; issue: "2";
pages: "315-319"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Published
Only in Database (2007)"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical Genetics
Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL
:http://cgl.riken.go.jp/"
COMMENT SGRef: number: 4; type: "Journal Article"
COMMENT Fission yeast expression vector.
FEATURES Location/Qualifiers
source 1..5347
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator 21..208
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
misc_feature 242..956
/pseudo
/gene="lys1"
/label=derived from Schizosaccharomyces pombe
/note="derived from Schizosaccharomyces pombe"
CDS 242..956
/pseudo
/codon_start=1
/gene="lys1"
/label=lys1
/note="NotI site is created at the middle of this fragment
(613-620)."
/translation="VLCSTCAVSRSNIFGIAKRCHSSRTWHEECPTFGDQSF*Y*QDMR
YRRSW*NISSCWWIG*GLPWK**ID*QKILKELVC*SLQVCRPNSRKCALETLLVRNTR
SYVSFW*LGPLSPNW*CRM*RPPTIKLKFVVSELS*VKLIPIFLVILMSVKILPLFDVI
RMKNPR*LHTLYLKV*IKMTLIALLSRKILSSMV*RSTGSLYMIYGNILRLNFLVMLFL
L*LFPFIKCLSILMER"
gene 242..956
/pseudo
/gene="lys1"
/label=lys1
promoter 962..1369
/label=TEF1 promoter
/note="promoter for EF-1-alpha"
promoter 1376..1423
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 1442..1837
/codon_start=1
/label=BSD
/note="blasticidin S deaminase"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
terminator 1934..2181
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(2207..2223)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 2436..2891
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3173..3277
/label=AmpR promoter
CDS 3278..4135
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4309..4897
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 5185..5206
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5221..5251
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5259..5275
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5283..5299
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
This page is informational only.