Basic Vector Information
- Vector Name:
- pLX-Z4-PmasRFP-ALCR
- Antibiotic Resistance:
- Gentamycin
- Length:
- 7308 bp
- Type:
- Cloning vector
- Replication origin:
- oriV
- Source/Author:
- Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez D, Garcia JA.
- Promoter:
- MAS
pLX-Z4-PmasRFP-ALCR vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLX-Z4-PmasRFP-ALCR vector Sequence
LOCUS 40924_29446 7308 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pLX-Z4-PmasRFP-ALCR, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7308)
AUTHORS Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez
D, Garcia JA.
TITLE Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors
with Compatible Replication Origins
JOURNAL ACS Synth Biol (2017) In press
PUBMED 28657330
REFERENCE 2 (bases 1 to 7308)
AUTHORS Pasin F, Garcia JA.
TITLE Direct Submission
JOURNAL Submitted (26-MAR-2017) Department of Plant Molecular Genetics,
Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid,
Madrid 28049, Spain
REFERENCE 3 (bases 1 to 7308)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7308)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
Biol (2017) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(26-MAR-2017) Department of Plant Molecular Genetics, Centro
Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid
28049, Spain"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7308
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 27..58
/regulatory_class="terminator"
misc_feature 59..83
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
misc_feature complement(106..129)
/note="left border of left T-DNA; LB"
promoter 179..559
/label=MAS promoter
/note="mannopine synthase promoter (Velten et al., 1984)"
CDS 570..1283
/label=TagRFP-T
/note="photostable variant of TagRFP (Shaner et al., 2008)"
mat_peptide 1281..1355
/product="T2A"
CDS 1302..1355
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 1356..3818
/gene="alcR"
/label=alcR
/note="Regulatory protein alcR from Emericella nidulans
(strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
M139). Accession#: P21228"
terminator 3824..4076
/label=MAS terminator
/note="mannopine synthase terminator"
misc_feature 4131..4154
/label=RB
/note="RB"
regulatory complement(4225..4277)
/regulatory_class="terminator"
CDS complement(4341..4871)
/label=GmR
/note="gentamycin acetyltransferase"
promoter complement(5060..5088)
/label=Pc promoter
/note="class 1 integron promoter"
misc_feature 5089..5112
/label=assembly linker
/note="assembly linker"
CDS complement(5166..6311)
/label=trfA
/note="trans-acting replication protein that binds to and
activates oriV"
rep_origin complement(6704..7235)
/direction=LEFT
/label=oriV
/note="incP origin of replication"
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