Basic Vector Information
- Vector Name:
- pLX-R2-TagRFP-T
- Antibiotic Resistance:
- Kanamycin
- Length:
- 6185 bp
- Type:
- Cloning vector
- Replication origin:
- oriV
- Host:
- Plants
- Source/Author:
- Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez D, Garcia JA.
- Promoter:
- CaMV 35S (enhanced)
pLX-R2-TagRFP-T vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLX-R2-TagRFP-T vector Sequence
LOCUS 40924_29391 6185 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pLX-R2-TagRFP-T, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6185)
AUTHORS Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez
D, Garcia JA.
TITLE Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors
with Compatible Replication Origins
JOURNAL ACS Synth Biol (2017) In press
PUBMED 28657330
REFERENCE 2 (bases 1 to 6185)
AUTHORS Pasin F, Garcia JA.
TITLE Direct Submission
JOURNAL Submitted (26-MAR-2017) Department of Plant Molecular Genetics,
Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid,
Madrid 28049, Spain
REFERENCE 3 (bases 1 to 6185)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6185)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
Biol (2017) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(26-MAR-2017) Department of Plant Molecular Genetics, Centro
Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid
28049, Spain"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6185
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..24
/label=assembly linker
/note="assembly linker"
regulatory 25..72
/regulatory_class="terminator"
misc_feature 133..157
/label=LB T-DNA repeat
/note="left border repeat from octopine Ach5 T-DNA"
misc_feature 184..208
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
promoter 535..1204
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
5'UTR 1204..1349
/label=PPV
/note="PPV"
CDS 1350..2060
/label=TagRFP-T
/note="photostable variant of TagRFP (Shaner et al., 2008)"
3'UTR 2236..2452
/label=PPV
/note="PPV"
terminator 2534..2786
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 2851..2874
/label=RB
/note="RB"
regulatory complement(2939..2979)
/regulatory_class="terminator"
misc_feature 2989..3012
/label=assembly linker
/note="assembly linker"
CDS complement(3026..3838)
/label=KanR
/note="aminoglycoside phosphotransferase"
misc_feature 3940..3963
/label=assembly linker
/note="assembly linker"
CDS complement(4017..5162)
/label=trfA
/note="trans-acting replication protein that binds to and
activates oriV"
rep_origin complement(5555..6086)
/direction=LEFT
/label=oriV
/note="incP origin of replication"
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