pLX-PPV vector (V004885)

LOCUS       40924_29386       14938 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLX-PPV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14938)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA.
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins
  JOURNAL   ACS Synth Biol (2017) In press
  PUBMED    28657330
REFERENCE   2  (bases 1 to 14938)
  AUTHORS   Pasin F, Garcia JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2017) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 14938)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14938)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAR-2017) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..24
                     /label=assembly linker
                     /note="assembly linker"
     regulatory      25..72
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        514..1183
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     5'UTR           1183..1328
     mat_peptide     1329..2252
                     /product="P1"
     mat_peptide     2253..3626
                     /product="HCPro"
     intron          4107..4295
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     mat_peptide     4866..5021
                     /product="6K1"
     mat_peptide     5022..6926
                     /product="CI"
     mat_peptide     6927..7085
                     /product="6K2"
     mat_peptide     7086..8393
                     /product="NIa"
     mat_peptide     8394..9947
                     /product="NIb"
     CDS             9951..10667
                     /label=EGFP
                     /note="enhanced GFP"
     mat_peptide     10692..11681
                     /product="CP"
     3'UTR           11685..11901
     terminator      12014..12266
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    12304..12327
                     /label=RB
                     /note="RB"
     regulatory      complement(12392..12432)
                     /regulatory_class="terminator"
     misc_feature    12442..12465
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(12479..13291)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    13393..13416
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(13420..14079)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(14080..14851)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"