pLX-CaMV vector (V004886)

LOCUS       V004886                12857 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004886
VERSION     V004886
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12857)
  AUTHORS   Pasin F, Tseng XA, Bedoya LC, Heydarnejad J, Deng TC, Garcia JA,
            Chen YR.
  TITLE     Streamlined generation of plant virus infectious clones using the
            pLX mini binary vectors
  JOURNAL   J. Virol. Methods (2018) In press
   PUBMED   30236898
REFERENCE   2  (bases 1 to 12857)
  AUTHORS   Pasin F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2018) Agricultural Biotechnology Research Center,
            Academia Sinica, 128 Academia Road, Section 2, Nangang District,
            Taipei, Taipei 11529, Taiwan
REFERENCE   3  (bases 1 to 12857)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12857)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: UltraCycler v. v1.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.
            Methods (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-APR-2018) Agricultural Biotechnology Research Center, Academia
            Sinica, 128 Academia Road, Section 2, Nangang District, Taipei,
            Taipei 11529, Taiwan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      25..72
                     /label="bacterial terminator"
                     /note="bacterial terminator"
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label="LB T-DNA repeat"
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        535..1212
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     polyA_signal    1251..1427
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2168..3151
                     /codon_start=1
                     /product="movement protein"
                     /label="movement protein"
                     /protein_id="AYC81468.1"
                     /translation="MDLYPEENTQSEQSQNSENNMQIFKSENSDGFSSDLMISNDQLKN
                     ISKTQLTLEKEKIFKMPNVLSQVMKKAFSRKNEIHYCVSTKELSVDIHDATGKVYLPLI
                     TKEEINKRLSSLKPEVRKTMSMVHLGAVKILLKAQFRNGIDTPIKIALIDDRINSRKDC
                     LLGAAKGNLAYGKFMFTVYPKFGISLNTQRLNQTLSLIHDFENKNLMNKGDKVMTITYI
                     VGYALTNSHHSIDYQSNATIELEDVFQEIGNVQQSDFCTIQNDECNWAIDIAQNKALLG
                     AKAKTQIGNSLQIGNGASSSNTENELARVSQNIDLLKNKLKEICGE"
     CDS             3154..3633
                     /codon_start=1
                     /product="aphid transmission protein"
                     /label="aphid transmission protein"
                     /protein_id="AYC81469.1"
                     /translation="MSITGQPHVYKKDTIIRLKPLSLNSNNRSYVFSSSKGNIQNIINH
                     LNNLNEIVGRSLLGIWKINSYFGLSKDPSESKSKNPSVFNTAKTIFKSGGVDYSSQLKE
                     IKSLLESQNTRIKSLEKAIQSLDDKIEPEPLTKEEVKELKESINSIKEGLKNIIG"
     CDS             3635..4021
                     /note="Virion-associated protein from Cauliflower mosaic
                     virus (strain Strasbourg). Accession#: P03551"
                     /label="Virion-associated protein"
     CDS             4006..5469
                     /codon_start=1
                     /product="capsid protein"
                     /label="capsid protein"
                     /protein_id="AYC81471.1"
                     /translation="MAESILDRTINRFWYNLGEDCLSESQFDLMIRLMEESLDGDQIID
                     LTSLPSDNLQVEQVMTTTEDSISEESEFLLAIGETSEDESDSGEEPEFEQVRMDRTGGT
                     EIPKEEDGEPSRYNERKRKTPEDRYFPTQPKTIPGQKQTSMGMLNIDCQTNRRTLIDDW
                     AAEIGLIVKTNREDYLDPETILLLMEHKTSGIAKELIRNTRWNRTTGDIIEQVIDAMYT
                     MFLGLNYSDNKVAEKIDEQEKAKIRMTKLQLCDICYLEEFTCDYEKNMYKTELADFPGY
                     INQYLSKIPIIGEKALTRFRHEANGTSTYSLGFAAKIVKEELSKICDLSKKQKKLKKFN
                     KKCCSIGEASVEYGCKKTSKKKYHKRYKKKYKVYKPYKKKKKFRSGKYFKPKEKKGSKQ
                     KYCPKGKKDCRCWICNIEGHYANECPNRQSSEKAHILQQAENLGLQPIEEPYEGVQEVF
                     ILEYKEEEEETSTEESDDGSSTSEDSDSD"
     CDS             5432..7468
                     /note="Enzymatic polyprotein from Cauliflower mosaic virus
                     (strain Strasbourg). Accession#: P03554"
                     /label="Enzymatic polyprotein"
     promoter        8891..9236
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    9281..9457
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     regulatory      9903..10158
                     /label="nos terminator"
                     /note="nos terminator"
                     /regulatory_class="terminator"
     terminator      9906..10158
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10223..10246
                     /label="RB"
                     /note="RB"
     regulatory      10311..10351
                     /label="bacterial terminator"
                     /note="bacterial terminator"
                     /regulatory_class="terminator"
     CDS             complement(10398..11210)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(11339..11998)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(11999..12770)
                     /direction=LEFT
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"