Basic Vector Information
- Vector Name:
- pLX-CaMV
- Antibiotic Resistance:
- Kanamycin
- Length:
- 12857 bp
- Type:
- Binary vector
- Replication origin:
- pBBR1 oriV
- Host:
- Plants
- Source/Author:
- Pasin F, Tseng XA, Bedoya LC, Heydarnejad J, Deng TC, Garcia JA, Chen YR.
- Promoter:
- CaMV 35S (enhanced)
pLX-CaMV vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLX-CaMV vector Sequence
LOCUS 40924_29381 12857 bp DNA circular SYN 18-DEC-2018
DEFINITION Binary vector pLX-CaMV, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12857)
AUTHORS Pasin F, Tseng XA, Bedoya LC, Heydarnejad J, Deng TC, Garcia JA,
Chen YR.
TITLE Streamlined generation of plant virus infectious clones using the
pLX mini binary vectors
JOURNAL J. Virol. Methods (2018) In press
PUBMED 30236898
REFERENCE 2 (bases 1 to 12857)
AUTHORS Pasin F.
TITLE Direct Submission
JOURNAL Submitted (11-APR-2018) Agricultural Biotechnology Research Center,
Academia Sinica, 128 Academia Road, Section 2, Nangang District,
Taipei, Taipei 11529, Taiwan
REFERENCE 3 (bases 1 to 12857)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 12857)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.
Methods (2018) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(11-APR-2018) Agricultural Biotechnology Research Center, Academia
Sinica, 128 Academia Road, Section 2, Nangang District, Taipei,
Taipei 11529, Taiwan"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Assembly Method :: UltraCycler v. v1.0
Sequencing Technology :: Illumina
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..12857
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 25..72
/label=bacterial terminator
/note="bacterial terminator"
/regulatory_class="terminator"
misc_feature 133..157
/label=LB T-DNA repeat
/note="left border repeat from octopine Ach5 T-DNA"
misc_feature 184..208
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
promoter 535..1212
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
polyA_signal 1251..1427
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS 2168..3151
/codon_start=1
/product="movement protein"
/label=movement protein
/protein_id="AYC81468.1"
/translation="MDLYPEENTQSEQSQNSENNMQIFKSENSDGFSSDLMISNDQLKN
ISKTQLTLEKEKIFKMPNVLSQVMKKAFSRKNEIHYCVSTKELSVDIHDATGKVYLPLI
TKEEINKRLSSLKPEVRKTMSMVHLGAVKILLKAQFRNGIDTPIKIALIDDRINSRKDC
LLGAAKGNLAYGKFMFTVYPKFGISLNTQRLNQTLSLIHDFENKNLMNKGDKVMTITYI
VGYALTNSHHSIDYQSNATIELEDVFQEIGNVQQSDFCTIQNDECNWAIDIAQNKALLG
AKAKTQIGNSLQIGNGASSSNTENELARVSQNIDLLKNKLKEICGE"
CDS 3154..3633
/codon_start=1
/product="aphid transmission protein"
/label=aphid transmission protein
/protein_id="AYC81469.1"
/translation="MSITGQPHVYKKDTIIRLKPLSLNSNNRSYVFSSSKGNIQNIINH
LNNLNEIVGRSLLGIWKINSYFGLSKDPSESKSKNPSVFNTAKTIFKSGGVDYSSQLKE
IKSLLESQNTRIKSLEKAIQSLDDKIEPEPLTKEEVKELKESINSIKEGLKNIIG"
CDS 3635..4021
/note="Virion-associated protein from Cauliflower mosaic
virus (strain Strasbourg). Accession#: P03551"
CDS 4006..5469
/codon_start=1
/product="capsid protein"
/label=capsid protein
/protein_id="AYC81471.1"
/translation="MAESILDRTINRFWYNLGEDCLSESQFDLMIRLMEESLDGDQIID
LTSLPSDNLQVEQVMTTTEDSISEESEFLLAIGETSEDESDSGEEPEFEQVRMDRTGGT
EIPKEEDGEPSRYNERKRKTPEDRYFPTQPKTIPGQKQTSMGMLNIDCQTNRRTLIDDW
AAEIGLIVKTNREDYLDPETILLLMEHKTSGIAKELIRNTRWNRTTGDIIEQVIDAMYT
MFLGLNYSDNKVAEKIDEQEKAKIRMTKLQLCDICYLEEFTCDYEKNMYKTELADFPGY
INQYLSKIPIIGEKALTRFRHEANGTSTYSLGFAAKIVKEELSKICDLSKKQKKLKKFN
KKCCSIGEASVEYGCKKTSKKKYHKRYKKKYKVYKPYKKKKKFRSGKYFKPKEKKGSKQ
KYCPKGKKDCRCWICNIEGHYANECPNRQSSEKAHILQQAENLGLQPIEEPYEGVQEVF
ILEYKEEEEETSTEESDDGSSTSEDSDSD"
CDS 5432..7468
/note="Enzymatic polyprotein from Cauliflower mosaic virus
(strain Strasbourg). Accession#: P03554"
promoter 8891..9236
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
polyA_signal 9281..9457
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
regulatory 9903..10158
/label=nos terminator
/note="nos terminator"
/regulatory_class="terminator"
terminator 9906..10158
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 10223..10246
/label=RB
/note="RB"
regulatory 10311..10351
/label=bacterial terminator
/note="bacterial terminator"
/regulatory_class="terminator"
CDS complement(10398..11210)
/label=KanR
/note="aminoglycoside phosphotransferase"
CDS complement(11339..11998)
/label=pBBR1 Rep
/note="replication protein for the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica"
rep_origin complement(11999..12770)
/direction=LEFT
/label=pBBR1 oriV
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"
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