Basic Vector Information
- Vector Name:
- pLX-B2-PetohmNEON
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5007 bp
- Type:
- Cloning vector
- Replication origin:
- pBBR1 oriV
- Source/Author:
- Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez D, Garcia JA.
pLX-B2-PetohmNEON vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLX-B2-PetohmNEON vector Sequence
LOCUS 40924_29326 5007 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pLX-B2-PetohmNEON, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5007) AUTHORS Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez D, Garcia JA. TITLE Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins JOURNAL ACS Synth Biol (2017) In press PUBMED 28657330 REFERENCE 2 (bases 1 to 5007) AUTHORS Pasin F, Garcia JA. TITLE Direct Submission JOURNAL Submitted (26-MAR-2017) Department of Plant Molecular Genetics, Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 28049, Spain REFERENCE 3 (bases 1 to 5007) TITLE Direct Submission REFERENCE 4 (bases 1 to 5007) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth Biol (2017) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (26-MAR-2017) Department of Plant Molecular Genetics, Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 28049, Spain" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5007 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..24 /label=assembly linker /note="assembly linker" regulatory 25..72 /regulatory_class="terminator" misc_feature 133..157 /label=LB T-DNA repeat /note="left border repeat from octopine Ach5 T-DNA" misc_feature 184..208 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" regulatory 260..449 /label=CaMV 35S /note="CaMV 35S" /regulatory_class="terminator" regulatory 450..804 /note="alcA; FMV 34S" /regulatory_class="promoter" misc_feature 826..881 /label=TMV Omega /note="translational enhancer from the tobacco mosaic virus 5'-leader sequence (Gallie et al., 1988)" CDS 883..1590 /label=mNeonGreen /note="bright monomeric yellow-green fluorescent protein derived from LanYFP (Shaner et al., 2013)" 3'UTR 1758..1974 terminator 2056..2308 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" misc_feature 2373..2396 /label=RB /note="RB" regulatory complement(2461..2501) /regulatory_class="terminator" misc_feature 2511..2534 /label=assembly linker /note="assembly linker" CDS complement(2548..3360) /label=KanR /note="aminoglycoside phosphotransferase" misc_feature 3462..3485 /label=assembly linker /note="assembly linker" CDS complement(3489..4148) /label=pBBR1 Rep /note="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" rep_origin complement(4149..4920) /direction=LEFT /label=pBBR1 oriV /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication"
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