pLX-B2-PetohmNEON vector (V004897)

Basic Vector Information

Vector Name:
pLX-B2-PetohmNEON
Antibiotic Resistance:
Kanamycin
Length:
5007 bp
Type:
Cloning vector
Replication origin:
pBBR1 oriV
Source/Author:
Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez D, Garcia JA.

pLX-B2-PetohmNEON vector Vector Map

pLX-B2-PetohmNEON5007 bp6001200180024003000360042004800assembly linkerLB T-DNA repeatLB T-DNA repeatCaMV 35SalcA; FMV 34STMV OmegamNeonGreen3'UTRNOS terminatorRBassembly linkerKanRassembly linkerpBBR1 ReppBBR1 oriV

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pLX-B2-PetohmNEON vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_29326        5007 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLX-B2-PetohmNEON, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA.
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins
  JOURNAL   ACS Synth Biol (2017) In press
  PUBMED    28657330
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Pasin F, Garcia JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2017) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 5007)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5007)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAR-2017) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..24
                     /label=assembly linker
                     /note="assembly linker"
     regulatory      25..72
                     /regulatory_class="terminator"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      260..449
                     /label=CaMV 35S
                     /note="CaMV 35S"
                     /regulatory_class="terminator"
     regulatory      450..804
                     /note="alcA; FMV 34S"
                     /regulatory_class="promoter"
     misc_feature    826..881
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             883..1590
                     /label=mNeonGreen
                     /note="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
     3'UTR           1758..1974
     terminator      2056..2308
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2373..2396
                     /label=RB
                     /note="RB"
     regulatory      complement(2461..2501)
                     /regulatory_class="terminator"
     misc_feature    2511..2534
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(2548..3360)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    3462..3485
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(3489..4148)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(4149..4920)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"

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