pLV.MCS.WHVPRE vector (V004910)

LOCUS       V004910                 6271 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004910
VERSION     V004910
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Ramkisoensing AA, Swildens J, Atsma DE, Pijnappels DA, Schalij MJ,
            de Vries AAF.
  TITLE     Gap junctional coupling with cultured neonatal rat cardiomyocytes
            induces the cardiomyogenic differentiation of fetal human
            mesenchymal stem cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Ramkisoensing AA, Swildens J, Atsma DE, Pijnappels DA, Schalij MJ,
            de Vries AAF.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2011) Cardiology, Leiden University Medical
            Center, Albinusdreef 2, Leiden, Zuid-Holland 2333 ZA, The
            Netherlands
REFERENCE   3  (bases 1 to 6271)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6271)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-AUG-2011) Cardiology, Leiden University Medical Center,
            Albinusdreef 2, Leiden, Zuid-Holland 2333 ZA, The Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1799..1916
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     misc_feature    1917..2053
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     misc_feature    2058..2646
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             2785..3018
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3090..3224
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      3251..3386
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(3407..3425)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3435..3451)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3596..4051
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4080..4184
                     /label="AmpR promoter"
     CDS             4185..5042
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5216..5804
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6092..6113
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6129..6159
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6167..6183
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6191..6207
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6228..6246
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"