Basic Vector Information
- Vector Name:
- pLV.MCS.WHVPRE
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6271 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Ramkisoensing AA, Swildens J, Atsma DE, Pijnappels DA, Schalij MJ, de Vries AAF.
- Promoter:
- RSV
pLV.MCS.WHVPRE vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLV.MCS.WHVPRE vector Sequence
LOCUS V004910 6271 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004910
VERSION V004910
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 6271)
AUTHORS Ramkisoensing AA, Swildens J, Atsma DE, Pijnappels DA, Schalij MJ,
de Vries AAF.
TITLE Gap junctional coupling with cultured neonatal rat cardiomyocytes
induces the cardiomyogenic differentiation of fetal human
mesenchymal stem cells
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6271)
AUTHORS Ramkisoensing AA, Swildens J, Atsma DE, Pijnappels DA, Schalij MJ,
de Vries AAF.
TITLE Direct Submission
JOURNAL Submitted (26-AUG-2011) Cardiology, Leiden University Medical
Center, Albinusdreef 2, Leiden, Zuid-Holland 2333 ZA, The
Netherlands
REFERENCE 3 (bases 1 to 6271)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6271)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(26-AUG-2011) Cardiology, Leiden University Medical Center,
Albinusdreef 2, Leiden, Zuid-Holland 2333 ZA, The Netherlands"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6271
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 3..229
/label="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
LTR 230..410
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 457..582
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1075..1308
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1493..1537
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1686..1727
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 1799..1916
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
misc_feature 1917..2053
/label="multiple cloning site"
/note="multiple cloning site"
misc_feature 2058..2646
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 2785..3018
/label="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 3090..3224
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 3251..3386
/label="SV40 ori"
/note="SV40 origin of replication"
promoter complement(3407..3425)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3435..3451)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 3596..4051
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4080..4184
/label="AmpR promoter"
CDS 4185..5042
/label="AmpR"
/note="beta-lactamase"
rep_origin 5216..5804
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 6092..6113
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6129..6159
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 6167..6183
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6191..6207
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 6228..6246
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
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