Basic Vector Information
- Vector Name:
- pLuxR-wt
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 5157 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
pLuxR-wt vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLuxR-wt vector Sequence
LOCUS 40924_28851 5157 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pLuxR-wt, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5157)
AUTHORS Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
TITLE A synthetic multicellular system for programmed pattern formation
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5157)
AUTHORS Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
TITLE Direct Submission
JOURNAL Submitted (21-JAN-2005) Electrical Engineering, Princeton
University, J-319, Engineering Quadrangle, Olden Street, Princeton,
NJ 08544, USA
REFERENCE 3 (bases 1 to 5157)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5157)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(21-JAN-2005) Electrical Engineering, Princeton University, J-319,
Engineering Quadrangle, Olden Street, Princeton, NJ 08544, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5157
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(125..1204)
/label=lacI
/note="lac repressor"
RBS 1208..1216
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 1428..2177
/gene="luxR"
/label=luxR
/note="Transcriptional activator protein LuxR from
Aliivibrio fischeri. Accession#: P12746"
terminator 2291..2377
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
protein_bind 2502..2523
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2538..2568
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2576..2592
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2600..2616
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
RBS 2681..2692
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 2699..3412
/label=yeGFP
/note="yeast-enhanced green fluorescent protein"
rep_origin complement(3571..4159)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator complement(4247..4341)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(4365..5021)
/label=CmR
/note="chloramphenicol acetyltransferase"
promoter complement(5022..5124)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
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