Basic Vector Information
- Vector Name:
- pLR5a
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6751 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Koksharov MI, Ugarova NN.
pLR5a vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLR5a vector Sequence
LOCUS V004946 6751 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004946
VERSION V004946
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 6751)
AUTHORS Koksharov MI, Ugarova NN.
TITLE Fast and simple in vivo photographic assessment of luciferase
properties in E. coli cells to screen and compare mutants of beetle
luciferases
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6751)
AUTHORS Koksharov MI.
TITLE Direct Submission
JOURNAL Submitted (21-FEB-2016) Department of Chemical Enzymology, Faculty
of Chemistry, Lomonosov Moscow State University, Leninskie gory,
Bld. 1/11, Moscow 119991, Russia
REFERENCE 3 (bases 1 to 6751)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6751)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(21-FEB-2016) Department of Chemical Enzymology, Faculty of
Chemistry, Lomonosov Moscow State University, Leninskie gory, Bld.
1/11, Moscow 119991, Russia"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6751
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(8..757)
/gene="luxR"
/label="Transcriptional activator protein LuxR"
/note="Transcriptional activator protein LuxR from
Aliivibrio fischeri. Accession#: P12746"
CDS 976..1554
/gene="luxI"
/label="Acyl-homoserine-lactone synthase"
/note="Acyl-homoserine-lactone synthase from Aliivibrio
fischeri. Accession#: P12747"
primer_bind 1584..1611
/label="F_start_pLRx"
/note="F_start_pLRx"
CDS 1615..3252
/note="Luciferin 4-monooxygenase from Luciola mingrelica.
Accession#: Q26304"
/label="Luciferin 4-monooxygenase"
CDS 3265..3282
/label="6xHis"
/note="6xHis affinity tag"
terminator 3349..3396
/label="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 3433..3888
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3915..4019
/label="AmpR promoter"
CDS 4020..4877
/label="AmpR"
/note="beta-lactamase"
rep_origin 5051..5639
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(5825..5967)
/label="bom"
/note="basis of mobility region from pBR322"
CDS complement(6072..6260)
/label="rop"
/note="Rop protein, which maintains plasmids at low copy
number"
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