pLP11 vector (V004954)

LOCUS       40924_28631        4500 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Suicide vector pLP11, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Luo P, He X, Liu Q, Hu C.
  TITLE     Developing Universal Genetic Tools for Rapid and Efficient Deletion 
            Mutation in Vibrio Species Based on Suicide T-Vectors Carrying a 
            Novel Counterselectable Marker, vmi480
  JOURNAL   PLoS ONE 10 (12), E0144465 (2015)
  PUBMED    26641275
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Luo P, He X, Hu C, Liu Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2015) Key Laboratory of Marine Bio-resources 
            Sustainable Utilization, South China Sea Institute of Oceanology, 
            Xingang Xi Road 164, Guangzhou, Guangdong 510301, China
REFERENCE   3  (bases 1 to 4500)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4500)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "12"; pages: "E0144465"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUL-2015) Key Laboratory of Marine Bio-resources Sustainable 
            Utilization, South China Sea Institute of Oceanology, Xingang Xi 
            Road 164, Guangzhou, Guangdong 510301, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..86
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     promoter        complement(92..110)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(125..513)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(641..1297)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1298..1400)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        1527..1545
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     oriT            1699..1808
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        2079..2156
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             2157..3236
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     protein_bind    3252..3273
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      3441..3475
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     promoter        3790..3818
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    3826..3842
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3901..4497
                     /codon_start=1
                     /product="toxin vmi480"
                     /label=toxin vmi480
                     /protein_id="ALS55901.1"
                     /translation="MTKKPEFYAPDLTEERLHILSENLLDVLDEAHHYSESPNATAWFK
                     GTANYGLPQGMLIRMHSDSAYPWLTLANQTMDYTARVGNTLVQFVVDDPHSPRKQHRLK
                     RNAVEKHQISLELEEDYVDTPLIWRFYLNPISNGVDYSPSISLLGFNGNGNVICSWEYD
                     TVVTGPVVTDKPESVEIDEPLLVRKKKVQKKVSDE"