pLES003 vector (V005019)

LOCUS       V005019                 6134 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005019
VERSION     V005019
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Wada T, Noda M, Kashiwabara F, Jeon HJ, Shirakawa A, Yabu H, Matoba
            Y, Kumagai T, Sugiyama M.
  TITLE     Characterization of four plasmids harboured in a Lactobacillus
            brevis strain encoding a novel bacteriocin, brevicin 925A, and
            construction of a shuttle vector for lactic acid bacteria and
            Escherichia coli
  JOURNAL   Microbiology (Reading, Engl.) 155 (PT 5), 1726-1737 (2009)
   PUBMED   19372160
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   Noda M, Sugiyama M.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-DEC-2007) Contact:Masafumi Noda Hiroshima University,
            Graduate School of Biomedical Sciences; Kasumi 1-2-3, Minami-ku,
            Hiroshima 734-8553, Japan
REFERENCE   3  (bases 1 to 6134)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6134)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Microbiology (Reading, Engl.) 155 (PT 5), 1726-1737 (2009)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-DEC-2007) Contact:Masafumi Noda Hiroshima University, Graduate
            School of Biomedical Sciences; Kasumi 1-2-3, Minami-ku, Hiroshima
            734-8553, Japan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             299..1033
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     primer_bind     1576..1592
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    1593..1649
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1662..1678)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1686..1702)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1710..1740)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1755..1776)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2064..2652)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2826..3683)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3684..3788)
                     /label="AmpR promoter"
     CDS             complement(4357..5280)
                     /codon_start=1
                     /gene="repA"
                     /product="plasmid replication protein"
                     /label="repA"
                     /protein_id="BAH56454.1"
                     /translation="MSNELVKYQPELNTIPLRKFSPTEMNLFFSIISRMRDKGDQTVRF
                     SFDQLKDLSNYKPTANRRFIDDLKRTYNHLMDLRFGSQSKSGLSFERFVMFTKFKINGD
                     VEEPYVDIEIYKDALPLLNHLESWVRYALVEFRDLKSSYAKTMFRLLKGYRTTGYAYFS
                     KSDFIELLDIPKSYRQSNTDQYVISPIKEELTPLFRGLTVRKKYGKGRGKPVIGYSFAW
                     KPERKDAEDVHVSKTERLKKAKFNIEHNGELSDKEKWRAIDKIKGLKLGTTEAEHNKQE
                     QEAREEQIRAEERQKILEKLRNGGLR"
     gene            complement(4357..5280)
                     /gene="repA"
                     /label="repA"
     rep_origin      complement(5336..5509)
                     /direction=LEFT
                     /note="replication origin in Gram-positive bacteria (iteron
                     region)"