Basic Vector Information
- Vector Name:
- pLeo695
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8537 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M.
- Promoter:
- EF-1α
pLeo695 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLeo695 vector Sequence
LOCUS 40924_28142 8537 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pLeo695, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8537)
AUTHORS Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M.
TITLE Generalized extracellular molecule sensor platform for programming
cellular behavior
JOURNAL Nat. Chem. Biol. 14 (7), 723-729 (2018)
PUBMED 29686358
REFERENCE 2 (bases 1 to 8537)
AUTHORS Scheller L, Strittmatter T.
TITLE Direct Submission
JOURNAL Submitted (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel
4058, Switzerland
REFERENCE 3 (bases 1 to 8537)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8537)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
Biol."; date: "2018"; volume: "14"; issue: "7"; pages: "723-729"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058,
Switzerland"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8537
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 3..1184
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
misc_feature 1200..1204
/label=SfiI
/note="SfiI"
sig_peptide 1243..1305
/label=Ig-kappa leader
/note="leader sequence from mouse immunoglobulin kappa
light
chain"
misc_feature 1312..1689
/label=anti RR120
/note="anti RR120"
misc_feature 1697..2445
/label=EpoR-D1_D2_TM
/note="EpoR-D1_D2_TM"
misc_feature 1975..1977
/label=F93A
/note="F93A"
misc_feature 2455..3283
/label=IL6ST
/note="IL6ST"
misc_feature 2806..2808
/label=Y759A
/note="Y759A"
polyA_signal 3380..3604
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
misc_feature complement(3653..3672)
/label=itr
/note="itr"
promoter 4036..4140
/label=AmpR promoter
CDS 4141..4998
/label=AmpR
/note="beta-lactamase"
rep_origin 5172..5760
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 6187..6206
/label=itr
/note="itr"
polyA_signal complement(6323..6378)
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
CDS complement(6546..7142)
/label=PuroR
/note="puromycin N-acetyltransferase"
misc_feature complement(7143..7202)
/label=P2A
/note="P2A"
CDS complement(7143..7199)
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS complement(7209..7907)
/label=TagBFP
/note="monomeric blue fluorescent protein"
regulatory complement(7921..8532)
/label=RPBSA
/note="RPBSA"
/regulatory_class="promoter"
regulatory 7921..8532
/label=RPBSA
/note="RPBSA"
/regulatory_class="promoter"
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