Basic Vector Information
- Vector Name:
- pLeo694
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8969 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M.
- Promoter:
- EF-1α
pLeo694 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLeo694 vector Sequence
LOCUS 40924_28137 8969 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pLeo694, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8969)
AUTHORS Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M.
TITLE Generalized extracellular molecule sensor platform for programming
cellular behavior
JOURNAL Nat. Chem. Biol. 14 (7), 723-729 (2018)
PUBMED 29686358
REFERENCE 2 (bases 1 to 8969)
AUTHORS Scheller L, Strittmatter T.
TITLE Direct Submission
JOURNAL Submitted (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel
4058, Switzerland
REFERENCE 3 (bases 1 to 8969)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8969)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
Biol."; date: "2018"; volume: "14"; issue: "7"; pages: "723-729"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058,
Switzerland"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8969
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 3..1184
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
misc_feature 1200..1204
/label=SfiI
/note="SfiI"
regulatory 1237..1246
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 1243..1305
/label=igk secretion signal
/note="igk secretion signal"
misc_feature 1306
/label=scfv
/note="scfv"
misc_feature 1312..1689
/label=anti RR120
/note="anti RR120"
misc_feature 1702..2445
/label=mouse EpoR-D1_D2_TM
/note="mouse EpoR-D1_D2_TM"
misc_feature 1975..1977
/label=F93A
/note="F93A"
misc_feature 2446..3717
/label=mFGFR 405-end
/note="mFGFR 405-end"
polyA_signal 3812..4036
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
misc_feature complement(4085..4104)
/label=itr
/note="itr"
promoter 4468..4572
/label=AmpR promoter
CDS 4573..5430
/label=AmpR
/note="beta-lactamase"
rep_origin 5604..6192
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 6619..6638
/label=itr
/note="itr"
polyA_signal complement(6755..6810)
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
CDS complement(6978..7574)
/label=PuroR
/note="puromycin N-acetyltransferase"
misc_feature complement(7575..7634)
/label=P2A
/note="P2A"
CDS complement(7575..7631)
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS complement(7641..8339)
/label=TagBFP
/note="monomeric blue fluorescent protein"
regulatory complement(8353..8964)
/label=RPBSA
/note="RPBSA"
/regulatory_class="promoter"
regulatory 8353..8964
/label=RPBSA
/note="RPBSA"
/regulatory_class="promoter"
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