Basic Vector Information
- Vector Name:
- pLeo667
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7371 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M.
- Promoter:
- SV40
pLeo667 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLeo667 vector Sequence
LOCUS 40924_28097 7371 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pLeo667, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7371)
AUTHORS Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M.
TITLE Generalized extracellular molecule sensor platform for programming
cellular behavior
JOURNAL Nat. Chem. Biol. 14 (7), 723-729 (2018)
PUBMED 29686358
REFERENCE 2 (bases 1 to 7371)
AUTHORS Scheller L, Strittmatter T.
TITLE Direct Submission
JOURNAL Submitted (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel
4058, Switzerland
REFERENCE 3 (bases 1 to 7371)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7371)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
Biol."; date: "2018"; volume: "14"; issue: "7"; pages: "723-729"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058,
Switzerland"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7371
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 7..215
/label=Psv40
/note="Psv40"
promoter 18..214
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 65..200
/label=SV40 ori
/note="SV40 origin of replication"
regulatory 240..249
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 246..308
/label=igk secretion signal
/note="igk secretion signal"
misc_feature 315..662
/label=Nic_VH
/note="Nic_VH"
misc_feature 675..1418
/label=mouse EpoR-D1_D2_TM
/note="mouse EpoR-D1_D2_TM"
misc_feature 948..950
/label=F93A
/note="F93A"
misc_feature 1350..1418
/label=transmembrane
/note="transmembrane"
misc_feature 1419..2690
/label=mFGFR 405-end
/note="mFGFR 405-end"
polyA_signal 2744..2968
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3014..3442
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3456..3785
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 3852..4643
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 4820..4953
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4990..5006)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5014..5030)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5038..5068)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5083..5104)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5392..5977)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6151..7008)
/label=AmpR
/note="beta-lactamase"
promoter complement(7009..7113)
/label=AmpR promoter
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