Basic Vector Information
- Vector Name:
- pLacCOs1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9287 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Oster CJ, Phillips GJ.
pLacCOs1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLacCOs1 vector Sequence
LOCUS 40924_27533 9287 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pLacCOs1, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9287)
AUTHORS Oster CJ, Phillips GJ.
TITLE Vectors for ligation-independent construction of lacZ gene fusions
and cloning of PCR products using a nicking endonuclease
JOURNAL Plasmid 66 (3), 180-185 (2011)
PUBMED 21854804
REFERENCE 2 (bases 1 to 9287)
AUTHORS Oster C, Phillips GJ.
TITLE Direct Submission
JOURNAL Submitted (26-APR-2011) Veterinary Microbiology, Iowa State
University, 1802 University Boulevard, Ames, IA 50010, USA
REFERENCE 3 (bases 1 to 9287)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9287)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
date: "2011"; volume: "66"; issue: "3"; pages: "180-185"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(26-APR-2011) Veterinary Microbiology, Iowa State University, 1802
University Boulevard, Ames, IA 50010, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9287
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 70..3114
/label=lacZ
/note="beta-galactosidase"
CDS 3169..4419
/gene="lacY"
/label=lacY
/note="Lactose permease from Escherichia coli (strain K12).
Accession#: P02920"
CDS 5982..6170
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
misc_feature 6275..6415
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(6601..7189)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7363..8220)
/label=AmpR
/note="beta-lactamase"
promoter complement(8221..8325)
/label=AmpR promoter
terminator 8452..8538
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 8633..8719
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 8814..8900
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator complement(9000..9043)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
misc_feature 9174..9230
/label=MCS
/note="pUC18/19 multiple cloning site"
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