LITMUS28 vector (V012565)

MSDS

Basic Vector Information

- - Vector Name:
  - LITMUS28
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 2823 bp
- - Type:
  - Cloning Vectors
- - Replication origin:
  - ori
- - Source/Author:
  - Evans PD, Cook SN, Riggs PD, Noren CJ.
- - Copy Number:
  - High copy number
- - Promoter:
  - lac
- - 5' Primer:
  - M13 fwd
- - 3' Primer:
  - M13 rev

LITMUS28 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

LITMUS28 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       LITMUS28.        2823 bp DNA     circular SYN 01-JAN-1980
DEFINITION  High copy-number E. coli vector with a multiple cloning site flanked
            by mutant T7 promoters. For wild-type T7 promoters, use LITMUS28i.
ACCESSION   .
VERSION     .
KEYWORDS    LITMUS28.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Evans PD, Cook SN, Riggs PD, Noren CJ.
  TITLE     LITMUS: multipurpose cloning vectors with a novel system for 
            bidirectional in vitro transcription.
  JOURNAL   BioTechniques 1995;19:130-5.
  PUBMED    7669286
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 2823)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1995"; volume: "19"; pages: "130-5"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        38..142
                     /label=AmpR promoter
     CDS             143..1000
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(1045..1554)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      1665..2253
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2314..2335
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2350..2380
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2388..2404
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2412..2428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2449..2466
                     /note="T7 promoter (mutant)"
                     /note="mutant version of the promoter for bacteriophage T7
                     RNA polymerase"
     misc_feature    2460..2608
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(2603..2620)
                     /note="T7 promoter (mutant)"
                     /note="mutant version of the promoter for bacteriophage T7
                     RNA polymerase"
     primer_bind     complement(2624..2640)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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