Basic Vector Information
- Vector Name:
- LITMUS38i
- Antibiotic Resistance:
- Ampicillin
- Length:
- 2814 bp
- Type:
- Cloning Vectors
- Replication origin:
- ori
- Source/Author:
- Evans PD, Cook SN, Riggs PD, Noren CJ.
- Copy Number:
- High copy number
- Cloning Method:
- Enzyme Cut
- 5' Primer:
- M13 fwd
- 3' Primer:
- M13 rev
LITMUS38i vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
LITMUS38i vector Sequence
LOCUS LITMUS38i. 2814 bp DNA circular SYN 01-JAN-1980
DEFINITION High copy-number E. coli vector with a multiple cloning site flanked
by wild-type T7 promoters. For an alternative MCS, use LITMUS28i.
ACCESSION .
VERSION .
KEYWORDS LITMUS38i.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2814)
AUTHORS Evans PD, Cook SN, Riggs PD, Noren CJ.
TITLE LITMUS: multipurpose cloning vectors with a novel system for
bidirectional in vitro transcription.
JOURNAL BioTechniques 1995;19:130-5.
PUBMED 7669286
REFERENCE 2 (bases 1 to 2814)
AUTHORS New England Biolabs
TITLE Direct Submission
REFERENCE 3 (bases 1 to 2814)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"BioTechniques"; date: "1995"; volume: "19"; pages: "130-5"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..2814
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 38..142
/label=AmpR promoter
CDS 143..1000
/label=AmpR
/note="beta-lactamase"
rep_origin complement(1045..1554)
/direction=LEFT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
rep_origin 1665..2253
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2314..2335
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2350..2380
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2388..2404
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2412..2428
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 2449..2467
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 2467..2579
/gene="lacZalpha"
/label=MCS
/note="MCS"
/note="multiple cloning site"
promoter complement(2593..2611)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2615..2631)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
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