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pMCS5 vector (V012445)

Price Information

Cat No. Plasmid Name Availability Add to cart
V012445 pMCS5 In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

Vector Name:
pMCS5
Antibiotic Resistance:
Ampicillin
Length:
3081 bp
Type:
Cloning Vectors
Replication origin:
ori
Source/Author:
MoBiTec
Copy Number:
High copy number
Promoter:
T7
5' Primer:
M13 fwd
3' Primer:
M13 rev

pMCS5 vector Vector Map

pMCS53081 bp6001200180024003000M13 fwdMCST7 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterf1 ori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMCS5 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pMCS5.        3081 bp DNA     circular SYN 01-JAN-1980
DEFINITION  E. coli cloning vector with an extensive multiple cloning site 
            containing 59 unique restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    pMCS5.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   MoBiTec
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    168..426
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(429..447)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(462..478)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(486..502)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(510..540)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(555..576)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(864..1452)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1626..2483)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2484..2588)
                     /label=AmpR promoter
     rep_origin      2615..3043
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"