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pNEB193 vector (V012442)

Basic Vector Information

Vector Name:
pNEB193
Antibiotic Resistance:
Ampicillin
Length:
2713 bp
Type:
Cloning Vectors
Replication origin:
ori
Source/Author:
New England Biolabs
Copy Number:
High copy number
5' Primer:
M13 fwd
3' Primer:
M13 rev

pNEB193 vector Vector Map

pNEB1932713 bp600120018002400M13 fwdMCSM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNEB193 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pNEB193.        2713 bp DNA     circular SYN 01-JAN-1980
DEFINITION  pUC19 derivative with additional sites for the 8-cutters AscI, PmeI,
            and PacI.
ACCESSION   .
VERSION     .
KEYWORDS    pNEB193.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    396..479
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     primer_bind     complement(492..508)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(516..532)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(540..570)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(585..606)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(894..1482)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1656..2513)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2514..2618)
                     /label=AmpR promoter

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