pTrcHis B vector (V012382)

Basic Vector Information

      • Vector Name:
      • pTrcHis B
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 4404 bp
      • Type:
      • Cloning Vectors
      • Source/Author:
      • Invitrogen (Life Technologies)
      • Copy Number:
      • High copy number

pTrcHis B vector Vector Map

pTrcHis B4404 bp600120018002400300036004200trc promoterlac operatorrrnG antiterminatorgene 10 translational enhancerRBSminicistronATG6xHisT7 tag (gene 10 leader)Xpress(TM) tagMCSrrnB T1 terminatorrrnB T2 terminatorAmpR promoterAmpRoribomlacIq promoterlacI

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTrcHis B vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pTrcHis_B.        4404 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for inducible expression of N-terminally 
            6xHis-tagged proteins. For other reading frames, use pTrcHis A or 
            pTrcHis C.
ACCESSION   .
VERSION     .
KEYWORDS    pTrcHis B.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        193..222
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    263..332
                     /label=rrnG antiterminator
                     /note="antiterminator from the E. coli rrnG leader region
                     (Berg et al., 1989)"
     misc_feature    346..354
                     /label=gene 10 translational enhancer
                     /note="gene 10 translational enhancer"
                     /note="translational enhancer from gene 10 of bacteriophage
                     T7 (Olins and Rangwala, 1989)"
     RBS             369..373
                     /note="ribosome binding site"
     CDS             383..406
                     /label=minicistron
                     /note="synthetic cistron containing a ribosome binding site
                     (Shine-Dalgarno sequence), for enhancing the bacterial 
                     expression of a downstream cistron (Schoner, 1997)"
     CDS             413..415
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             425..442
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             446..478
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             482..505
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     misc_feature    505..554
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      757..843
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      935..962
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        982..1073
                     /label=AmpR promoter
     CDS             1074..1931
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2105..2693
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2879..3019)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        3205..3282
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3283..4362
                     /label=lacI
                     /note="lac repressor"

This page is informational only.