Basic Vector Information
pTV 118N vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTV 118N vector Sequence
LOCUS 40924_44504 3163 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial cloning and expression vector with an NcoI site overlapping the lacZ-alpha start codon. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3163) AUTHORS TaKaRa TITLE Direct Submission REFERENCE 2 (bases 1 to 3163) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..3163 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 233..254 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 269..299 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 307..323 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." misc_feature 358..414 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(418..434) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin complement(829..1284) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 1384..1488 /label=AmpR promoter CDS 1489..2346 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 2520..3108 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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