Basic Vector Information
pTWV228 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTWV228 vector Sequence
LOCUS 40924_44539 4039 bp DNA circular SYN 01-JAN-1980
DEFINITION Low copy number bacterial vector for cloning sequences that cause
toxicity.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4039)
AUTHORS TaKaRa
TITLE Direct Submission
REFERENCE 2 (bases 1 to 4039)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4039
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(187..642)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 855..871
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature complement(875..931)
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(941..957)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(965..981)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(989..1019)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(1034..1055)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS 1601..1789
/codon_start=1
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
/translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
misc_feature 1894..2034
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(2220..2808)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2982..3839)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3840..3944)
/label=AmpR promoter
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