Basic Vector Information
pTZ19U vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTZ19U vector Sequence
LOCUS 40924_44624 2862 bp DNA circular SYN 01-JAN-1980 DEFINITION Phagemid vector constructed by inserting the phage f1 ori and the T7 promoter into pUC19. The f1 ori direction is reversed in pTZ19R. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2862) AUTHORS Thermo Scientific (Fermentas) TITLE Direct Submission REFERENCE 2 (bases 1 to 2862) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..2862 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(1..456) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 598..614 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 615..671 /label=MCS /note="pUC18/19 multiple cloning site" promoter complement(674..692) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(711..727) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(735..751) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(759..789) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(804..825) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1113..1701) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1875..2732) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2733..2837) /label=AmpR promoter
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