pTZ57R vector (V012371)

MSDS

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V012371	pTZ57R	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

This is a cloning vector with blue/white screening, suitable for generating ExoIII deletions. Derived from pTZ19R.

- - Vector Name:
  - pTZ57R
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 2886 bp
- - Type:
  - Cloning Vectors
- - Replication origin:
  - ori
- - Source/Author:
  - Thermo Scientific (Fermentas)
- - Copy Number:
  - High copy number
- - 5' Primer:
  - M13 fwd
- - 3' Primer:
  - M13 rev

pTZ57R vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

Rezaee H, Salehi M, Bandehpour M, Kalantari S, Hosseini S, Agin K, Kazemi B. Production of CFTR Mutant Gene Model by Homologous Recombination System. Cell J. 2022 Oct 1;24(10):596-602.

pTZ57R vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pTZ57R.        2886 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Cloning vector with blue/white screening, suitable for generating 
            ExoIII deletions. Derived from pTZ19R.
ACCESSION   .
VERSION     .
KEYWORDS    pTZ57R.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Thermo Scientific (Fermentas)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    615..695
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(698..716)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(735..751)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(759..775)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(783..813)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(828..849)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1137..1725)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1899..2756)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2757..2861)
                     /label=AmpR promoter