Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V012370 | pUAST | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
pUAST vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUAST vector Sequence
LOCUS pUAST. 8897 bp DNA circular SYN 01-JAN-1980 DEFINITION P element-based vector for Gal4-regulated expression of genes in Drosophila. ACCESSION . VERSION . KEYWORDS pUAST. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8897) AUTHORS Brand AH, Perrimon N. TITLE Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. JOURNAL Development 1993;118:401-15. PUBMED 8223268 REFERENCE 2 (bases 1 to 8897) AUTHORS Brand Lab TITLE Direct Submission REFERENCE 3 (bases 1 to 8897) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Development"; date: "1993"; volume: "118"; pages: "401-15" COMMENT SGRef: number: 2; type: "Journal Article" COMMENT http://www.gurdon.cam.ac.uk/~brandlab/3.html FEATURES Location/Qualifiers source 1..8897 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..46 /label=MCS /note="MCS" /note="multiple cloning site" intron 124..189 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 319..339 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" polyA_signal 611..745 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" gene 763..4899 /label=mini-white /note="This modified version of the white gene lacks part of the first intron." misc_feature complement(4910..5495) /label=P element 5' end misc_feature 5496..5724 /label=white linker sequence /note="white linker sequence" rep_origin complement(5730..6318) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6492..7349) /label=AmpR /note="beta-lactamase" promoter complement(7350..7454) /label=AmpR promoter misc_feature 7755..8255 /label=white linker sequence /note="white linker sequence" misc_feature complement(8256..8488) /label=P element 3' end /note="P element 3' end" protein_bind 8533..8627 /label=5X UAS /note="five tandem copies of the 'ScaI site' 17-mer CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) that efficiently binds yeast Gal4 (Webster et al., 1988; Pfeiffer et al., 2010)" promoter 8646..8884 /label=hsp70 promoter /note="Drosophila melanogaster hsp70Bb promoter"