Basic Vector Information
pUC118 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUC118 vector Sequence
LOCUS 40924_44953 3162 bp DNA circular SYN 01-JAN-1980 DEFINITION Cloning vector with a phage origin for producing single-stranded DNA. The MCS is reversed in pUC119. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3162) AUTHORS Vieira J, Messing J. TITLE Production of single-stranded plasmid DNA. JOURNAL Meth. Enzymol. 1987;153:3-11. PUBMED 3323803 REFERENCE 2 (bases 1 to 3162) AUTHORS TaKaRa TITLE Direct Submission REFERENCE 3 (bases 1 to 3162) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Meth. Enzymol."; date: "1987"; volume: "153"; pages: "3-11" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..3162 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(187..642) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 855..871 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature complement(875..931) /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(941..957) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(965..981) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(989..1019) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(1034..1055) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1343..1931) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2105..2962) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2963..3067) /label=AmpR promoter
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