pUC118 vector (V012369)

Basic Vector Information

      • Vector Name:
      • pUC118
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 3162 bp
      • Type:
      • Cloning Vectors
      • Source/Author:
      • Vieira J, Messing J.
      • Copy Number:
      • High copy number
      • 5' Primer:
      • M13 fwd
      • 3' Primer:
      • M13 rev

pUC118 vector Vector Map

pUC1183162 bp6001200180024003000f1 oriM13 fwdMCSM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUC118 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_44953        3162 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Cloning vector with a phage origin for producing single-stranded 
            DNA. The MCS is reversed in pUC119.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Vieira J, Messing J.
  TITLE     Production of single-stranded plasmid DNA.
  JOURNAL   Meth. Enzymol. 1987;153:3-11.
  PUBMED    3323803
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   TaKaRa
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3162)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Meth. 
            Enzymol."; date: "1987"; volume: "153"; pages: "3-11"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(187..642)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     855..871
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(875..931)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(941..957)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(965..981)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(989..1019)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1034..1055)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1343..1931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2105..2962)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2963..3067)
                     /label=AmpR promoter

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