Basic Vector Information
- Vector Name:
- pUC57-Simple
- Antibiotic Resistance:
- Ampicillin
- Length:
- 2710 bp
- Type:
- Cloning Vectors
- Replication origin:
- ori
- Source/Author:
- GenScript
- Copy Number:
- High copy number
- 5' Primer:
- M13 fwd
- 3' Primer:
- M13 rev
pUC57-Simple vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUC57-Simple vector Sequence
LOCUS 40924_45203 2710 bp DNA circular SYN 01-JAN-1980 DEFINITION Derivative of the standard cloning vector pUC57 with only an EcoRV site available for cloning. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2710) AUTHORS GenScript TITLE Direct Submission REFERENCE 2 (bases 1 to 2710) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..2710 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(489..505) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(513..529) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(537..567) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(582..603) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(891..1479) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1653..2510) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2511..2615) /label=AmpR promoter
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