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MLM3636 vector (V012322#)

Basic Vector Information

Basic Vector Information
Vector Name MLM3636 Antibiotic Resistance Ampicillin
Length 2278 bp Type CRISPR Plasmids
Source Joung Lab / Addgene #43860 Copy Number High copy number

MLM3636 vector Vector Map

U6 promoter gRNA scaffold polIII terminator ori bla bla

MLM3636 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                2278 bp ds-DNA    circular SYN 06-1-2016
DEFINITION  CRISPR/Cas9 plasmid for expressing a guide RNA (gRNA) from the U6 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    MLM3636
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Joung Lab / Addgene #43860
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     promoter        82..322
                     /note="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        350..425
                     /note="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      426..431
                     /note="polIII terminator"
                     /note="RNA polymerase III transcription terminator"
     rep_origin      complement(529..1117)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1288..2148)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2149..2253)
                     /gene="bla"
                     /note="AmpR promoter"

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