MLM3636 vector (V012322)

Basic Vector Information

      • Vector Name:
      • MLM3636
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 2278 bp
      • Type:
      • CRISPR Plasmids
      • Source/Author:
      • Joung Lab / Addgene #43860
      • Copy Number:
      • High copy number

MLM3636 vector Vector Map

MLM36362278 bp60012001800U6 promotergRNA scaffoldpolIII terminatororiAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

MLM3636 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                2278 bp ds-DNA    circular SYN 06-1-2016
DEFINITION  CRISPR/Cas9 plasmid for expressing a guide RNA (gRNA) from the U6 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    MLM3636
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Joung Lab / Addgene #43860
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     promoter        82..322
                     /note="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        350..425
                     /note="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      426..431
                     /note="polIII terminator"
                     /note="RNA polymerase III transcription terminator"
     rep_origin      complement(529..1117)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1288..2148)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2149..2253)
                     /gene="bla"
                     /note="AmpR promoter"

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