Basic Vector Information
MLM3636 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
MLM3636 vector Sequence
LOCUS MLM3636. 2278 bp DNA circular SYN 01-JAN-1980 DEFINITION CRISPR/Cas9 plasmid for expressing a guide RNA (gRNA) from the U6 promoter. ACCESSION . VERSION . KEYWORDS MLM3636. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2278) AUTHORS Joung Lab / Addgene #43860 TITLE Direct Submission REFERENCE 2 (bases 1 to 2278) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..2278 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 82..322 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" misc_RNA 350..425 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" terminator 426..431 /note="polIII terminator" /note="RNA polymerase III transcription terminator" rep_origin complement(529..1117) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1291..2148) /label=AmpR /note="beta-lactamase" promoter complement(2149..2253) /label=AmpR promoter
This page is informational only.