phKikGR1-S1 vector (V012001)

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V012001	phKikGR1-S1	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

- - Vector Name:
  - phKikGR1-S1
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 3366 bp
- - Type:
  - Fluorescent Protein Genes & Plasmids
- - Replication origin:
  - ori
- - Source/Author:
  - MBL International
- - Copy Number:
  - High copy number
- - 5' Primer:
  - M13 fwd
- - 3' Primer:
  - M13 rev

phKikGR1-S1 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

phKikGR1-S1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_24708        3366 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing hKikGR1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   MBL International
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2264..2947
                     /codon_start=1
                     /label=hKikGR1
                     /note="humanized photoconvertible Kikume green-to-red 
                     fluorescent protein"
                     /translation="MVSVITSEMKIELRMEGAVNGHKFVITGKGSGQPFEGIQNVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQSFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNCFVNEIRFDGVNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVNM
                     ALLLQGGGHYRCDFRTTYKAKKVVQLPDYHFVDHQMEITSHDKDYNKVKLYEHAKAHSG
                     LPRLAK"
     primer_bind     complement(2972..2988)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"