Basic Vector Information
phMGFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
phMGFP vector Sequence
LOCUS 40924_24732 4707 bp DNA circular SYN 18-DEC-2018 DEFINITION Monster GFP vector phMGFP, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4707) AUTHORS Sundquist T, Chauvin F, Almond B. TITLE Monster Green Fluorescent Protein phMGFP Vector Technical Bulletin, TB320 JOURNAL Unpublished REFERENCE 2 (bases 1 to 4707) AUTHORS Sundquist T, Chauvin F, Almond B. TITLE Direct Submission JOURNAL Submitted (14-JAN-2003) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA REFERENCE 3 (bases 1 to 4707) TITLE Direct Submission REFERENCE 4 (bases 1 to 4707) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (14-JAN-2003) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4707 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 139..517 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 518..721 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" intron 857..989 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" promoter 1034..1052 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1076..1753 /codon_start=1 /label=mc4 /note="mc4 is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2003, derived from Montastraea cavernosa." /translation="MGVIKPDMKIKLRMEGAVNGHKFVIEGDGKGKPFEGKQTMDLTVI EGAPLPFAYDILTTVFDYGNRVFAKYPKDIPDYFKQTFPEGYSWERSMTYEDQGICIAT NDITMMKGVDDCFVYKIRFDGVNFPANGPVMQRKTLKWEPSTEKMYVRDGVLKGDVNMA LLLEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHRIEIVSHDKDYNKVKLYEHAEAHSGL PRQA" promoter complement(1781..1798) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" polyA_signal complement(1816..1937) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 2123..2578 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2910..3014 /label=AmpR promoter CDS 3015..3872 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 4046..4634 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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