pmOrange2-C1 vector (V011964)

MSDS

Basic Vector Information

- - Vector Name:
  - pmOrange2-C1
- - Antibiotic Resistance:
  - Kanamycin
- - Length:
  - 4715 bp
- - Type:
  - Fluorescent Protein Genes & Plasmids
- - Replication origin:
  - ori
- - Source/Author:
  - Clontech
- - Selection Marker:
  - Neomycin/G418(Geneticin)
- - Copy Number:
  - High copy number

pmOrange2-C1 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pmOrange2-C1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pmOrange2-C1.        4715 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for fusing mOrange2 to the N-terminus of a partner protein.
ACCESSION   .
VERSION     .
KEYWORDS    pmOrange2-C1.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             606..1313
                     /label=mOrange2
                     /note="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
     misc_feature    1314..1379
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    1388..1398
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    1503..1624
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1631..2086)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2113..2217
                     /label=AmpR promoter
     promoter        2219..2576
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2611..3402
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3637..3684
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4013..4601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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