Basic Vector Information
Basic Vector Information | |||
---|---|---|---|
Vector Name | ptd-Tomato-N1 | Antibiotic Resistance | Kanamycin |
Length | 5443 bp | Type | Fluorescent Protein Genes & Plasmids |
Source | Clontech | Selection Marker | Neomycin/G418(Geneticin) |
Copy Number | High copy number |
ptd-Tomato-N1 vector Vector Map
Plasmid Resuspension protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
ptd-Tomato-N1 vector Sequence
LOCUS Exported 5443 bp ds-DNA circular SYN 04-1-2013 DEFINITION Vector for fusing tdTomato to the C-terminus of a partner protein. ACCESSION . VERSION . KEYWORDS ptd-Tomato-N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5443) AUTHORS Clontech TITLE Direct Submission COMMENT FEATURES Location/Qualifiers source 1..5443 /organism="synthetic DNA construct" /lab_host="Mammalian Cells" /mol_type="other DNA" enhancer 61..364 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" misc_feature 591..671 /note="MCS" /note="multiple cloning site of fluorescent protein plasmids" CDS 679..2109 /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative of DsRed" /note="tdTomato" /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE RSEGRHHLFLYGMDELYK" polyA_signal 2231..2352 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(2359..2814) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2841..2945 /gene="bla" /note="AmpR promoter" promoter 2947..3304 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 3155..3290 /note="SV40 ori" /note="SV40 origin of replication" CDS 3339..4133 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin)" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" misc_feature 4365..4412 /note="HSV TK poly(A) signal" /note="herpesvirus thymidine kinase polyadenylation signal" rep_origin 4741..5329 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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