Basic Vector Information
| Basic Vector Information | |||
|---|---|---|---|
| Vector Name | ptd-Tomato-N1 | Antibiotic Resistance | Kanamycin |
| Length | 5443 bp | Type | Fluorescent Protein Genes & Plasmids |
| Source | Clontech | Selection Marker | Neomycin/G418(Geneticin) |
| Copy Number | High copy number | ||
ptd-Tomato-N1 vector Vector Map
ptd-Tomato-N1 vector Sequence
LOCUS Exported 5443 bp ds-DNA circular SYN 04-1-2013
DEFINITION Vector for fusing tdTomato to the C-terminus of a partner protein.
ACCESSION .
VERSION .
KEYWORDS ptd-Tomato-N1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5443)
AUTHORS Clontech
TITLE Direct Submission
COMMENT
FEATURES Location/Qualifiers
source 1..5443
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 61..364
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 591..671
/note="MCS"
/note="multiple cloning site of fluorescent protein
plasmids"
CDS 679..2109
/codon_start=1
/product="tandem dimeric (pseudo-monomeric) derivative of
DsRed"
/note="tdTomato"
/note="mammalian codon-optimized"
/translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
RSEGRHHLFLYGMDELYK"
polyA_signal 2231..2352
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2359..2814)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2841..2945
/gene="bla"
/note="AmpR promoter"
promoter 2947..3304
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 3155..3290
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 3339..4133
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 4365..4412
/note="HSV TK poly(A) signal"
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 4741..5329
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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